摘要
目的:研究丹参注射液(SM)对庆大霉素(GM)耳中毒豚鼠耳蜗一氧化氮合酶(NOS)异构体表达的影响,探讨SM对GM耳毒性的防护机制。方法:40只豚鼠随机分成对照组、GM组、SM组和GM+SM组,应用SABC免疫组织化学方法及显微图像分析技术,观察NOS三型异构体在豚鼠耳蜗的表达;同时结合听脑干反应(ABR)测试,观察用药前后豚鼠听阈的变化。结果:GM+SM组豚鼠耳蜗诱导型NOS(iNOS/NOSⅡ)表达和ABR阈值均明显低于GM组(P<0.01);且iNOS表达变化与ABR阈值改变高度相关(|r|>0.7,P<0.01);而各组豚鼠耳蜗神经元型NOS(nNOS/NOSⅠ)和内皮型NOS(eNOS/NOSⅢ)表达均无显著性差异。结论:SM对GM耳中毒后豚鼠耳蜗nNOS和eNOS表达无影响,但可通过抑制GM所致iNOS高表达,以减少NO的过量生成,从而对GM的耳毒性损伤发挥防护作用。
Objective：To investigate the effects of salvia miltiorrhiza injection（SM） on gentamicin（GM）-induced expression of nitric o-xide synthase（NOS） isoforms in guinea pig cochlea,and to explore the protective mechanism of SM on GM-induced ototoxicity.Methods：40 guinea pigs were randomly assigned to 4 groups：control group,GM group,SM group and GM plus SM group.Expression of NOS isoforms in the guinea pig cochlea was detected by the SABC method of immunohistochemistry and microscope image analysis technique.Auditory threshold was tested by auditory brainstem response（ABR） measurement.Results：Inducible NOS（iNOS/NOSⅡ） expression and ABR threshold in GM plus SM group were both significantly declined as compared with those in GM group（P0.01）.Moreover,change of iNOS expression was in high correlation with that of ABR threshold（｜r｜0.7,P0.01）.While expression of neuronal NOS（nNOS/NOSⅠ） and endothelial NOS（eNOS / NOSⅢ） showed no significant differences in all groups.Conclusion：SM had no effect on the expression of nNOS and eNOS,but could inhibit iNOS high-expression induced by GM to reduce excessive generation of NO,therefore SM could protect against GM ototoxicity.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2011年第2期246-249,共4页
Chinese Journal of Applied Physiology
基金
辽宁省博士科研启动基金(20031086)
关键词
丹参注射液
庆大霉素
一氧化氮合酶
耳蜗
salvia miltiorrhiza injection
gentamicin
nitric oxide synthase
cochlea
