摘要
为了阐明草绿色链球菌（SV）体外对人精子运动的影响，先将不同浓度的活SV悬液、滤液以及经煮沸和青霉素处理的SV悬液在体外与10例健康成年生育男性的经上游优化处理的精子一起孵化，于孵化0、2、4和6h采用德国Mika计算机辅助的精子分析系统（CASA）分别测定具体外对人精子前向运动百分率的影响。结果显示：活SV悬液体外能明显降低前向运动精子百分率；活SV滤液、经煮沸和青霉素处理后的SV悬液体外对人精子前向运动精子百分率无明显影响。说明活的SV菌体本身体外能明显降低人前向运动精子百分率，但杀菌处理能消除其作用。
In order to investigate the influence of streptococcus viridans (SV) on human sperm progressive motiligy in vitro, Ejaculates were aseptically obtained by masturbation and prepared by swim-up technique to produce a spermatozoa solution of high motility from 10 healthy fertile men. The spermatozoa solution was incubated with the SV at 100, 10 and 1: 1 of the sperm: bacterial ratio respectively at 37℃ in vitro. Motion parameters were measured by an automatic analyzing system . Measurements were carried out at 0, 2, 4 and 6 h in all specimens. The results showed that the live-SV solutions with different concentrations could significantly affect human sperm progressive motility. The sperm agglutinations were observed. However, the filtrate of live-SV and the solution of dead-SV treated by boiling and penlcillin could not affect human sperm progressive motility. It is suggested that the live-SV can obviously decrease the human sperm motility in vitro. The boiling and penicillin treatment can eliminate the inhibitory effect of SV on human sperm progressive motility in vitro.
出处
《同济医科大学学报》
CSCD
1999年第6期512-514,共3页
Acta Universitatis Medicinae Tongji
关键词
草绿色链球菌
精子运动
计算机
streptococcus viridans
sperm motility
computer
