摘要
对经中能的１８ Ｏ８＋ 离子辐照过的胸腺嘧啶 Ｎ２ Ｏ 饱和水溶液进行了研究，利用气相色谱（ Ｇ Ｃ）、气相色谱质谱联用（ Ｇ Ｃ Ｍ Ｓ）、气相色谱红外光谱联用（ Ｇ Ｃ Ｆ Ｔ Ｉ Ｒ）等仪器对辐照后的产物进行了分析、鉴别，观察到了 ５，６二氢胸腺嘧啶、５羟基５甲基乙内酰脲、５羟基（氢）６（氢）羟基胸腺嘧啶、５羟甲基尿嘧啶、顺（反）胸腺嘧啶二醇和二聚体等产物。虽然重离子主要是通过辐照水溶液中产生的羟基、氢原子等对胸腺嘧啶分子产生了作用，但与 γ射线作用机制和结果是不同的。γ射线辐照后主要产生的是二聚体产物，而重离子辐照后主要产物却是羟基加成产物。
In this study some chromatographic methods with capillary column,such as GC, GC\|MS,GC\|FTIR,were used to analyze the products of thymine irradiated by 18 O 8+ ion beam in N\-2O saturated aqueous solution. Because of the complexity and small amounts of the products after irradiation by heavy ion beams, it is very difficult to separate them,while with the methods of capillary gas chromatography this problem can be overcome. From the results of GC\|MS analysis we can determine the molecular weight of the product, and from the results of GC\|FTIR analysis we can get structure information of the product. By this way the pro\| ducts such as 5,6\|dihydrothymine, 5\|hydroxyl\|5\|methylhydantoin, 5\|hydroxyl\|6\|hydrothymine, 5\|hydro\|6\|hydroxylthymine, 5\|hydroxymethyluracil, trans \|thymine glycol, cis \|thymine glycol and dimers were determined . Though these products are the same as those from thymine irradiated by γ ray in N\-2O saturated aqueous solution, the mechanism of heavy ion beam interact ion with thymine aqueous solution is different from that of γ ray. The main products from thymine irradiated by 18 O 8+ ion beam in N\-2O saturated aqueous solution are formed by hydroxyl additions between 5\|6 band of thymine, while those by γ ray are dimers of thymine.
出处
《色谱》
CAS
CSCD
北大核心
1999年第5期420-423,共4页
Chinese Journal of Chromatography
基金
中国科学院院长青年创新基金
关键词
毛细管气相色谱
重离子辐照
胸腺嘧啶
产物
capillary gas chromatography,irradiated by heavy ion beam, thymine
