摘要
目的:观察辅助性17 T细胞(Th17)及其分泌的细胞因子IL-17在自身免疫性溶血性贫血(AIHA)患者中的变化并探讨其临床意义。方法:应用流式细胞术(FCM)检测治疗前与糖皮质激素治疗后AIHA患者和健康人外周血中Th17细胞的比率,同时用ELISA法检测血浆中IL-17的水平。结果:治疗前AIHA患者外周血中Th17细胞比率和血浆中IL-17的水平分别为(2.78±0.59)%和(126.4±11.6)ng/L,较健康对照[分别为(0.59±0.15)%和(52.3±4.8)ng/L]显著增高(P<0.01);治疗后,外周血中Th17细胞比率和血浆中IL-17的水平[分别为(1.05±0.28)%和(78.5±6.4)ng/L]较治疗前下降(P<0.01)。结论:AIHA患者外周血中Th17细胞的比率及其IL-17的水平均高,用糖皮质激素治疗后可下调Th17细胞的比率及IL-17的水平,有效地缓解AIHA。
AIM： To observe the percentage of Th17 cells in the peripheral blood and levels of plasma IL-17 of patients with autoimmune hemolytic anemia（AIHA）before treatment and after glucocorticoid treatment and to explore their clinical significance.METHODS： Flow cytometric assay were used to detect the rate of Th17 cells in AIHA patients.The level of plasma IL-17 in AIHA patients were measured by ELISA.RESULTS： The rate of Th17 cells and the level of plasma IL-17 were significantly increased before treatment as compared with that in normal controls [（2.78±0.59）%,（126.4±11.6）ng/L vs（0.59±0.15）%,（52.3±4.8） ng/L]（P〈0.01）.After treatment,The percentage of Th17 cells（1.05±0.28）% was significantly decreased（P〈0.01）.The same tendency was also found in the levels of plasma IL-17（78.5±6.4） ng/L（P〈0.01）.CONCLUSION： The rate of Th17 cells and the lever of plasma IL-17 significantly elevate in AIHA patients.Glucocorticoid might play a role in AIHA treatment by down-regulating Th17 cells number and concentrations of IL-17.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2011年第6期662-663,667,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
上海市科学和技术委员会生物医药重大攻关课题专项研究基金资助项目(06DZ19013)
