摘要
为了提高碱性果胶酶基因工程茵(pWB980-pel521/WB600)产酶能力,实验室在摇瓶条件下采用Plackett-Burman(P-B)方法筛选出对产酶有重要影响的3个因素(豆饼粉、磷酸盐以及氯化钠的添加量),并采用响应面试验设计(RSM)对重要因素进行优化。结果表明,摇瓶发酵培养基成分为:麸皮30.00 g/L(浸汁)、豆饼粉35.3345g/L、磷酸盐0.04552 mol/L、氯化钠4.2988g/L、氯化钙1.11g/L的条件下,碱性果胶酶基因工程菌产酶能力得到了显著提高,酶活力达到约760 U/mL,产酶能力比发酵优化前提高了3倍。
In order to improve the alkaline pectate lyase（PEL） production in pWB980- pel521/WB600, three key factors such as the concentration of bean cake powder, phosphate and NaCI were selected by Plackett-Burrnan method through single factor experiment in shake flask. Meanwhile, response surface analysis was used to optimize these factors. The results showed that the optimal medium contained wheat bran 30.00 g/L, bean cake powder 35. 3345 g/L, phosphate 0. 04552 mol/L, NaCl 4. 2988 g/L, CaCl2 1.11 g/L. Under the optimized conditions, the alkaline pectate lyase activity was improved to 760 U/mL.
出处
《工业微生物》
CAS
CSCD
2011年第3期21-26,共6页
Industrial Microbiology
基金
教育部博士点基金项目资助(项目编号:200800570001)
