摘要
目的:研究黄芪提取物对小鼠生长性能、抗氧化及免疫功能的影响。方法:60只健康小鼠随机分为A、B、C三组,每组20只。A组为生理盐水对照组,B、C组为试验组,过对小鼠连续14 d灌胃黄芪提取物,称量小鼠的体重变化,测定肝脏SOD、CAT、GSH-Px活性和MDA浓度,计算小鼠胸腺指数和脾脏指数,并测定小鼠脾淋巴细胞增殖活性。结果:与对照组相比,试验组的小鼠体重,肝脏组织中SOD、CAT、GSH-Px的含量,小鼠的脾脏指数和胸腺指数,均有显著提高(P<0.05);肝脏中MDA水平显著降低;MTT比色法测定脾淋巴细胞增殖能力(OD值)与对照组相比,均显著提高(P<0.05)。结论:以活性成分黄芪多糖为主的黄芪提取物能显著增加小鼠体重和免疫器官的总质量,能明显提高机体免疫功能和抗氧化机能,并且对小鼠淋巴细胞增殖具有很强的促进作用。
Objective：To study the effect of extractive of astragalus on growing,antioxidation and immune function of mice. Methods：60 healthy rats were randomly divided into three groups（n=20,each）：blank control group（group A,with Saline Solution）,study group B,C（with astragalus root extract）.Mice were treated using stomach tube every day for 14 days,and their livers were taken to measure the weight,the activities of SOD,CAT GSH-Px and MDA.Their thymus and spleens were used to measure the index of thymus and spleens and the proliferation of splenic lymphocyte. Results：Compared with the blank control group,the results showed that weight,activities of SOD,CAT and GSH-Px,index of thymus and spleens,and the proliferation of splenic lymphocyte were increased significantly（P0.05）,but the MDA content was decreased,the proliferation of splenic lymphocyte（OD） were increased significantly（P0.05）,with MTT assay. Conclusions：The results showed that the extractive of astragalus can enhance the growing,antioxidant ability,and immune function of mice.
出处
《航空航天医学杂志》
2011年第2期144-145,147,共3页
Journal of Aerospace medicine
关键词
黄芪
抗氧化
胸腺指数
脾脏指数
淋巴细胞增殖
astragalus
antioxidant
index of thymus and spleens
the proliferation of splenic lymphocyte
