摘要
①目的筛选出一种适合提高刺五加苷含量的内生镰刀霉菌P109-4生长的培养基。②方法将P109-4接种于6种基础培养基中,30℃下培养,对其生长状况进行观察,并在12、24、36、48、60、72h时段测量菌落大小。筛选出一种长速率最快的的培养基。再对其进行改良找到能使P109-4生长最快的培养基。③结果 P109-4在6种基本培养中生长状况明显不同,30℃培养72h后内生菌在牛肉膏蛋白胨、高氏一号、查氏培养基、PDA培养基、乳糖牛肉膏蛋白胨培养基、LC培养基的生长直径分别为4.00、3.70、3.75、3.90、3.20、3.20、3.63 cm。在改良牛肉膏蛋白胨培养基试验中,培养72h后牛肉膏蛋白胨培养基、改良牛肉膏蛋白胨培养基1号、改良牛肉膏蛋白胨培养基2号生长直径分别为3.30、3.45、3.20 cm。④结论经过72h菌落直径的比较,刺五加内生镰刀霉菌在改良牛肉膏蛋白胨培养基1号,生长速率更快。
Objective In order to select a culture medium that is more adapted to the growth of endogenous fungis P109-4.Methods Meeting the endogenous fungi to 6 kinds of based culture mediums and culture in 30℃.According to observe the develop situation and measure the diameter of the clones in 12h、24 h、36 h、48 h、60 h、72 h,select which culture medium of endogenous fungis P109-4 develop fastest.Results The develop situation of endogenous fungis P109-4 is different in 6 culture medium,After 72 hours the colony diameters are 4.00cm、3.70cm、3.75 cm、3.90 cm、3.20 cm、3.20 cm、3.63 cm in 6 culture mediums.In beef extract and peptone,culture medium improved assay.After 72 hours,the colony diameters are 3.30 cm、3.45 cm、3.20cm in 3 culture mediums.Conclusion Compare of the colony diameters after 72 hours,the NO.1 improved beef extract and peptone culture medium is most adapted to the development of endogenous fungis of Eleutherococcus senticosus.
出处
《华北煤炭医学院学报》
2011年第3期299-300,共2页
Journal of North China Coal Medical College
基金
河北省自然科学基金(项目编号:C2009001252)
