摘要
目的:了解缺氧缺血性脑损伤(HIBD)新生大鼠心肌细胞凋亡和Calpain-1表达的变化。方法:采用改良的Rice法构建新生大鼠HIBD模型,64只大鼠随机分为对照组和HIBD后2、12、24 h及2、3、5、7 d组。应用TUNEL法检测心肌细胞凋亡情况,并计数凋亡指数(AI),RT-PCR法和Western blot法检测各组大鼠心肌中Calpain-1mRNA及蛋白活性的变化。结果:对照组偶见凋亡细胞,HIBD组凋亡细胞均明显增多(P<0.01),3 d达凋亡高峰(P<0.001),此后开始降低,7 d仍高于对照组(P<0.001)。与对照组相比,Calpain-1mRNA于HIBD后12 h开始增高(P<0.001),2 d达到高峰,3～5 d表达量仍维持在较高水平(P<0.001);蛋白活性自HIBD后2 h增高(P<0.05),3 d达高峰(P<0.001)后开始降低,7 d与对照组无统计学差异(P>0.05);Calpain-1 mRNA及蛋白活性均与AI呈直线正相关(r=0.786,P<0.01;r=0.853,P<0.01)。结论:缺氧缺血性脑损伤新生大鼠心肌中Calpain-1表达增强,且与细胞凋亡有关。
Objective： To detect the expressions of calpain-1mRNA and protein in myocardium of rats with hypoxic-ischemic brain damage（HIBD）.Methods： HIBD model was made by modified rice of 7-day-old Wistar rats,64 rats were randomly divided into eight groups： control group and groups with 2 h、12 h、24 h、2 d、3 d、5 d and 7 d of exposure to HIBD.Apoptosis index（AI） was detected by TUNEL.RT-PCR and Western blot were applied to detect the gene and protein expressions of calpain-1.Results：Apoptosis cells were found sporadically in control group and significantly higher in HIBD groups（P0.01）.AI was increased at 2 h and reached peak at 3 d,and it was decreased at 7 d but still more than that in control group（P0.01）.The expression of Calpain-1 mRNA began to increase at 12 h after HIBD and reached peak at 2 d（0.5400±0.0848,P0.01）,and then decreased but remained in a higher level than that of the control group until 5 d after HIBD（P0.01）;calpain-1 protein activation began to increase at 2 h after HIBD（P0.05） and reached peak at 3 d（P0.001）,and it was still in higher level at 5 d（P0.001） and same as the control at 7 d（P0.05）.The expressions of Calpain-1 mRNA and protein activation were positively correlated with AI（r=0.786,P0.01;r=0.853,P0.01）.Conclusion： Apoptosis cells and the expressions of calpain-1 are increased in myocardium of neonatal rats with HIBD,showing positive correlation with AI.
出处
《天津医科大学学报》
2011年第2期194-197,共4页
Journal of Tianjin Medical University
