摘要
目的:建立柴辛鼻敏康胶囊的质量标准。方法:采用薄层色谱法(TLC)对柴胡、细辛进行定性鉴别,采用高效液相色谱(HPLC)对阿魏酸进行含量测定。色谱柱:SHISEIDO C18(250 mm×4.6 mm,5μm),流动相:乙腈-0.1%磷酸溶液(15︰85),流速为1.0 mL.min-1,检测波长为320nm,柱温为30℃。结果:薄层色谱中,斑点清晰,易于识别;阿魏酸在0.0215～0.5370μg内线性良好(r=0.9999),平均回收率为99.84%,RSD 1.23%(n=6)。结论:该质量控制方法简单、专属性强,可有效控制柴辛鼻敏康胶囊的质量。
Objective： To establish the quality standards for quality control of Chaixinbiminkang capsule. Method： TLC was adopted to identify Bupleurum, Asarum; RP-HPLC method was used to test the content of Ferulic acid. The separation column SHISEIDO C18 （250 mm×4.6mm, 5 μm） was used with acetonitrile-0. 1% phosphoric acid （ 15： 85） as mobile phase. The flow rate was 1.0 ml · min-1. The detection wavelength was 320 rim, the column temperature was 30℃ Result： The spots in the TLC were clear and distinguished; Ferulic acid has good linearity relationship by HPLC in 0. 0215 ～ 0. 5370μg（r = 0. 999 9） , the average recovery is 99.84% , RSD is 1.23% （n = 6）. Conclusion： This method of qualitative control is precise,exclusive, and has good effect on quality control of Chaixinbiminkang capsule.
出处
《中国中医基础医学杂志》
CAS
CSCD
北大核心
2011年第6期676-677,共2页
JOURNAL OF BASIC CHINESE MEDICINE
