摘要
现有研究表明拟南芥中miR172通过参与光周期通路来调节植物的开花时间,其靶基因为APETALA2-Like基因,过表达miR172会导致植株提前开花.植物在恶劣环境影响下一般会提前开花,但miR172是否参与胁迫响应下的开花调节,目前还未见报道.通过启动子分析发现拟南芥miR172各个成员的启动子均包括多个胁迫响应元件,半定量RT-PCR表明miR172a-1受甘露醇和LiCl诱导.通过在拟南芥中分别过表达miR172a-1/b-2,转基因植株均比野生型侧根数量多,植株生长受抑不显著,说明它们可能参与了这2种胁迫的响应.半定量与电导率分析表明miR172c受干旱诱导.
microRNA172 mediates photoperiodic flowering in Arabidopsis and its targets are APETALA2-Like genes.Overexpression of miR172 results in early flowering.Although environmental stress causes the promotion of flowering,whether miR172 is involved in the flowering time under stress condition is unknown.The promoter regions of miR172 members all contain many different stress-responsive cis-acting elements.Semi-quantitative reverse transcription-PCR（RT-PCR） analysis indicates that mannitol or LiCl induces the expression of miR172a-1.The overexpression of miR172a-1 or miR172b-2 leads to more lateral roots,but there is no growth suppression comopared with the wild type.This implicates that they are involved in different stess responses respectively.RT-PCR and electrical conductivity analyses suggest that miR172 is induced under drought condition.
出处
《复旦学报（自然科学版）》
CAS
CSCD
北大核心
2011年第3期328-333,F0003,共7页
Journal of Fudan University：Natural Science
基金
国家自然科学基金青年基金资助项目(30900782)
上海市科委博士后基金(09R21411000)
新世纪优秀人才项目(NCET-07-0201)
上海市科委重点科技攻关资助项目(08391910400)
