摘要
目的:研究绿茶功能性成分——咖啡碱与茶多酚组合用于减肥的代谢机制。方法:将50只雌性昆明小鼠随机分成5组,即:对照组、0.03%咖啡碱+0.3%茶多酚、0.03%咖啡碱+0.6%茶多酚、0.06%咖啡碱+0.3%茶多酚及0.06%咖啡碱+0.6%茶多酚。投喂12周后解剖,摘取小鼠的肝及腹腔脂肪(IPAT)并称重。测定小鼠肝脏肉毒碱脂酰转移酶(CAT)、酰基辅酶A氧化酶(ACO)及脂肪酸合成酶(FAS)活性。结果:0.06%咖啡碱+0.6%茶多酚组小鼠的体重及体重增加与对照组相比显著降低。绿茶成分组合的腹腔脂肪系数明显低于对照组,CAT活性与对照组相比均显著提高。0.03%咖啡碱+0.6%茶多酚及0.06%咖啡碱+0.6%茶多酚组FAS活性显著降低。0.06%咖啡碱+0.6%茶多酚组的ACO活性与对照组相比显著提高。结论:绿茶功能性成分可通过抑制小鼠肝脏内脂肪酸合成,提高脂肪氧化,减少脂肪在体内沉积,达到减肥效果。
Objective：To investigate the effects of caffeine and catechins combination on mechanism of lipids metabolism in mice.Method：Fifty female kunming mice were randomly divided into 5 groups：control,0.03% caffeine ＋ 0.3% catechins,0.03% caffeine ＋ 0.6% catechins,0.06% caffeine ＋ 0.3% catechins and 0.06% caffeine ＋ 0.6% catechins for 12 weeks.Live and intraperitioneal adipose tissues （IPAT） were weighted at the end of this period.Carnitine acyltransferase （CAT）,acyl-CoA oxidase （ACO） and fatty acid synthase （FAS） activity in the liver were measured.Results：The body weight and body weight gain was significantly reduced by the diets containing 0.06% caffeine ＋ 0.6% catechins.The conbinations of green tea components significantly reduced the IPAT coefficient,increased the activity of CAT with compared to the control,and 0.06% caffeine ＋ 0.6% catechins can significantly increase the ACO activity,the FAS activity were significantly reduced by 0.03% caffeine ＋ 0.6% catechins and 0.06% caffeine ＋ 0.6% catechins.Conclusion：These results showed that the green tea components may reduce the activity of FAS,increase activities of CAT and ACO,it causes the suppressive effect on fat accumulation and body weight gain.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2011年第3期14-19,共6页
Journal of Chinese Institute Of Food Science and Technology
基金
江西省自然科学基金项目(No.2008GZN0024)
教育部留学回国人员科研启动基金项目(教外司留[2009]1001号)
江西省教育厅项目(No.GJJ10425)
关键词
咖啡碱与茶多酚组合
体重
腹腔脂肪
脂肪代谢
caffeine and catechins combination
body weight
intraperitioneal adipose tissues
lipids metabolism
