摘要
目的:对自行分离得到的耐热型植酸酶高产菌株芽孢杆菌ZJ0702发酵条件进行优化,以提高植酸酶的活力。方法:采用单因素试验,研究培养基组分和培养条件对该菌株产植酸酶活力的影响。结果:经优化得到的培养基组分为3.5%麸皮、2%蛋白胨、0.5%硝酸铵、0.01%无机磷、0.2%CaCl_2、0.05%KCl、0.03%MgSO_4、0.003%Fe-SO_4、0.003%MnSO_4、0.03%NaCl;最适培养条件为34℃,接种量7%,pH7.0,装液量75mL/250mL。在上述培养条件下该菌株发酵72h产植酸酶活力达到最高值,为11388.4U/mL。结论:与该菌株在原始条件下产酶活力8251U/mL相比,酶活提高了38%。
Objective:To optimize the fermention conditions of phytase by Bacillus nealsonii ZJ0702 which screened from soil in order to increase its activity.Methods:Effect of the medium components and culture conditions on phytase activity was studied using single-factor test.Results:The optimal fermention medium components(%) were:wheat bran 3.5%,tryptone 2%,KH2 PO4 0.01%,NH4NO3 0.5%,CaCl2 0.2%,KCl 0.05%,MgSO4 0.03%,FeSO4 0.003%,MnSO4 0.003%,NaCl 0.03%;the optimal culture conditions for enzyme-producing were:34 ℃,pH 7.0,inoculum volume 7% and liquid volume in flask 75 mL/250 mL.The phytase activity was up to 11 388.4 U/mL after the strain was cultured for 72 h under the above conditions.Conclusion:The phytase activity was 38% higher under the optimal conditions than that under the premitive conditions (8 251 U/mL).
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2011年第3期98-103,共6页
Journal of Chinese Institute Of Food Science and Technology
基金
浙江省新世纪151人才资助项目
关键词
耐热
芽孢杆菌
植酸酶
单因素优化
heat-resistant
Bacillus
phytase
single-factor optimization