摘要
目的建立SMP1基因外显子检测方法以探讨SMP1基因多态性。方法根据文献报道并参考Gene-Bank中的基因序列,设计聚合酶链式反应（PCR）扩增SMP1基因7个外显子的8对特异性引物,应用PCR分段扩增SMP1基因7个外显子,PCR产物纯化后直接测序,应用DNAMAN5.2.9序列分析软件分析序列。结果 SMP1基因第1-7外显子长度分别为137、106、113、68、93、60和1 703 bp。SMP1基因第1-6外显子未发现多态性位点,但该基因第7外显子存在1329T〉C和1704G〉A多态性。结论本研究成功建立SMP1基因外显子检测方法并检测到了SMP1基因第7外显子存在多态性位点。
Objective To establish the detection method of SMP1 gene exons and investigate the polymorphism of SMP1 gene.Methods A total of 7 exons of SMP1 gene were amplified by polymerase chain reaction（PCR） with 8 pairs of specific primers designed according to reported sequences from literatures and GeneBank.Amplicons were sequenced after being purified and the sequences were analyzed with DNAMAN5.2.9 software.Results The lengths of exons 1~7 of SMP1 gene were 137,106,113,68,93,60 and 1 703 bp,respectively.Polymorphism was not found in exons 1~6 of SMP1 gene,while 1329TC and 1704GA polymorphisms were found in exon 7.Conclusion Method for detecting the exons of SMP1 gene was set up successfully,and the results showed polymorphism in exon 7 of SMP1 gene.
出处
《中国输血杂志》
CAS
CSCD
北大核心
2011年第5期402-404,共3页
Chinese Journal of Blood Transfusion
