摘要
目的:考察叶酸-羧甲基壳聚糖-超顺磁氧化铁纳米粒(FA-OCMCS-SPIO-NPs)和羧甲基壳聚糖-超顺磁氧化铁纳米粒(OCMCS-SPIO-NPs)2种造影剂的急性毒性及其抗肝、脾吞噬的能力。方法:尾静脉给予小鼠2种造影剂低、中、高剂量(278、347.5、434.5mg(Fe)·kg-1)后观察14d内的一般情况,计算半数致死量(LD50),并进行各组织病理学检查;以葡聚糖-超顺磁氧化铁纳米粒(dextran-SPIO-NPs)为阳性对照,普鲁士蓝染色法观察2种造影剂经小鼠尾静脉注射相似剂量后抗肝、脾吞噬的能力。结果:2种造影剂的LD50>434.5mg(Fe)·kg-1,所有小鼠均未出现明显的毒性反应和组织病理学改变;普鲁士蓝染色切片结果显示,dextran-SPIO-NPs无法逃避肝、脾中巨噬细胞的吞噬,而FA-OCMCS-SPIO-NPs能完全逃避吞噬,OCMCS-SPIO-NPs大部分逃避了吞噬。结论:2种造影剂的安全性高,且能全身分布,具有潜在的肿瘤靶向载体价值。
OBJECTIVE： To evaluate the acute toxicity and the anti-phagocytosis of folic acid-O-carboxymethyl chitosans superparamagnetic iron oxide nanoparticles （FA-OCMCS-SPIO-NPs） and O-carboxymethyl chitosans superparamagnetic oxide iron nanoparticles （OCMCS-SPIO-NPs）.METHODS： We observed and recorded general information of mice during 14 days when they were injected with 2 kinds of contrast mediums low,medium and high dose （278,347.5,434.5 mg（Fe）·kg-1） from tail vein.The LD50 was calculated.The pathologic examination was prosecuted timely.Prussian blue stain was applied to illustrate the ability of double contrast mediums to escape from liver and spleen with dextran-SPIO-NPs as positive control.RESULTS： LD50 of two contrast mediums were all greater than 434.5 mg（Fe）·kg-1.Apparent toxic reaction and tissue injury did not find among all the animals.Prussian blue stain showed that FA-OCMCS-SPIO-NPs could escape from phagocytosis of liver and spleen completely and most of OCMCS-SPIO-NPs could while dextran-SPIO-NPs couldn’t.CONCLUSIONS： Two contrast mediums are safe and can distribute the whole body,so they possess potential value in tumor-targeting as carrier.
出处
《中国药房》
CAS
CSCD
北大核心
2011年第25期2344-2346,共3页
China Pharmacy
关键词
超顺磁氧化铁纳米粒
叶酸
羧甲基壳聚糖
小鼠
急性毒性
抗吞噬性
Superparamagnetic iron oxide nanoparticles
Folic acid
O-carboxymethyl chitosans
Mice
Acute toxicity
Anti-phagocytosis
