摘要
目的 观察 Ｋｉ６７ 抗原在胆囊良恶性肿瘤中的表达强度与肿瘤的分级分期、治疗效果和预后的关系。方法 采用免疫组织化学（ Ａ Ｐ Ａ Ａ Ｐ） 方法，用抗人 Ｋｉ６７ 抗原的等效抗体 Ｍ Ｉ Ｂ１ 比较研究 Ｋｉ６７ 蛋白在良恶性胆囊肿瘤和正常胆囊组织中的表达情况，以比较不同胆囊组织中细胞增殖的情况。结果 Ｋｉ６７ 蛋白在胆囊癌组织中表达的阳性指数率显著高于胆囊良性肿瘤及正常胆囊组织（ Ｐ＜ ０ ．０５） 。但 Ｋｉ６７ 蛋白的表达强度与胆囊癌的 Ｔ Ｎ Ｍ 分期及病理分级程度无相关关系。结论 Ｋｉ６７ 蛋白的阳性表达率对辅助临床病理、对胆囊恶性肿瘤的诊断有较高的实用价值。 Ｋｉ６７ 蛋白在胆囊癌中的高表达率也是其高度恶性的生物学行为的客观指标之一。
Objective\ To observe the expression of Ki 67 protein in benign and gallbladder neoplasm and normal gallbladder tissues and its relation to the tumor stage and grade, therapeutic effects and prognosis.Methods\ The expression of Ki 67 protein in benign and malignant gallbladder neoplasm was comparatively studied by using APAAP method and Ki 67 equivalent antibody, MIB 1. Results\ In gallbladder carcinoma, the expression rate of Ki 67 protein was significantly higher than in benign neoplasm and normal gallbladder tissue ( P <0.05). No correlation was found between the expression rate of Ki 67 and the TNM stages and pathological grades of gallbladder carcinoma.Conclusion\ The expression rate of Ki 67 protein is valuable for the differential diagnosis of gallbladder neoplasm clinically and pathologically. The higher expression of Ki 67 protein is also one of the points showing the higher malignancy of gallbladder carcinoma.\;
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
1999年第5期402-403,F002,共3页
Chinese Journal of Experimental Surgery
