摘要
应用疝修补术治疗腹壁疝在临床上得到广泛运用,不足之处是患者易受细菌感染。介绍高效固载缓释抗菌药物疝补材料的制备方法及其效果。利用复乳-溶剂挥发法,制备盐酸去甲万古霉素聚乳酸-羟基乙酸共聚物微球(NV-PLGA-MP);以乙烯-醋酸乙烯共聚物(EVA)为支撑剂,将该微球固载至聚丙烯(PP)补片,得到固载有盐酸去甲万古霉素聚乳酸-羟基乙酸共聚物微球的补片(NV-PLGA-MP-PP)。采用HPLC法,测定NV含量以及NV-PLGA-MP和NV-PLGA-MP-PP体外药物释放过程。用扫描电镜(SEM)图像进行形貌观测,结果表明,制备的微球表面光滑,NV-PLGA-MP-PP表面平整,载药量高;在实验时间范围内,NV-PLGA-MP释放NV达4 d,NV-PLGA-MP-PP释放NV可持续28 d以上,具有明显的缓释等优点。NV-PLGA-MP-PP载药量高,缓释时间长,符合理想的抗感染疝补片材料的性能要求。
Although abdominal hernia repair is widely used in clinic,the patients are susceptible to bacterial infection.This article proposes a method to prepare hernia mesh containing microspheres loaded norvancomycin（NV）.Microspheres loaded norvancomycin were prepared by double emulsion and solvent evaporation method.Moreover,the micrspheres were bonded to the polypropylene（PP） mesh by using ethylene-vinyl acetate copolymer as supporter to prepare NV-PP.The morphology was characterized by SEM and the content of norvancomycin of microspheres and in vitro release of NV-PP were measured by HPLC.The microspheres were maintained integrity and norvancomycin could be loaded into the microspheres with high loading efficiency.The resulted NV-PP＇s surface was roughness and sustained release profiles were observed.The drug loading of NV-PP is up to 50 mg/cm2,and the release profiles of the microparticles sustained over 28 days.The results suggested that NV-PP is a suitable anti-infective herian mesh.
出处
《中国生物医学工程学报》
CAS
CSCD
北大核心
2011年第3期433-440,共8页
Chinese Journal of Biomedical Engineering
基金
国家科技计划(2007BAK26B08)
天津市自然科学基金(09JCYBJC03500)
关键词
腹壁疝补片
去甲万古霉素微球
复乳-溶剂挥发法
abdominal hernia mesh
norvancomycin-loaded microspheres
double emulsion and solvent evaporation method
