摘要
目的探讨白细胞介素24(IL-24)对鼻咽癌CNE-2Z细胞株增殖的影响。方法取鼻咽癌CNE-2Z细胞株,进行细胞培养;用细胞计数、四甲基偶氮唑蓝(MTT)抑制试验,以IL-240 pg/L为对照组,检测不同浓度、不同时间的IL-24对鼻咽癌CNE-2Z细胞株增殖的影响。结果细胞计数及MTT比色法检测得出:体外培养的鼻咽癌CNE-2Z细胞株在培养的48 h增殖状况最差,并且随着IL-24浓度的增加,其活细胞数也随着减少,当IL-24为50 pg/L时,其增殖状况最差;当IL-24为100 pg/L时,其增殖状况反而好转。结论 IL-24可呈时间和剂量依赖性地抑制鼻咽癌CNE-2Z细胞株增殖。
Objective To investigate the effects of interleukin-24（IL-24）on proliferation of human nasopharyngeal carcinoma（NPC）cell strain CNE-2Z.Methods Cell-counting,methyl thiazolyl tetrazolium-inhibitory test were used to examine the inhibitory effect of IL-24 on the proliferation of NPC cell strain CNE-2Z.The experiment group was added IL-24 while the control group was not,so as to find the influence of IL-24 on NPC CNE-27 cell line proliferation at different concentrations and time.Results From cell-counting and MTT-inhibitory test： at 48 hours of the culture,NPC CNE-2Z cell line cultured in vitro grew worst.Living cells number decreased along with the increasing of IL-24 concentration,presenting the worst proliferation at the concentration of 50 pg/L of IL-24,then bounced back at the concentration of 100 pg/L.Conclusion IL-24 can decelerate NPC CNE-2Z cell line proliferation in a dose and time dependent manner.
出处
《医学综述》
2011年第12期1880-1882,共3页
Medical Recapitulate
