摘要
研究柞蚕(Antheraea pernyi)与栗蚕(Dictyoploca japonica)的人工授精技术,并应用于柞蚕与栗蚕的种间杂交试验,为利用远缘杂交改良柞蚕的经济性状提供试验技术。从种内交配40～60 min的柞蚕雌蛾交配囊中采集精子细胞用于对柞蚕处女蛾的人工授精,其受精蛾率为61.5%,受精卵率为70.5%,孵化卵率为60.8%。栗蚕精子可在种内交配40～75 min的雌蛾交配囊中采集。利用单蛾精液授精和单蛾与多蛾精液互补授精的方法,分别获得了栗蚕♀×柞蚕♂的越冬卵和蚁蚕及柞蚕♀×栗蚕♂的活胚胎。试验结果表明,采用初步建立的人工授精技术,可以进行柞蚕与栗蚕的远缘杂交。
In present study,the artificial insemination technique for tussah silkworm（Antheraea pernyi） and camphor silkworm（Dictyoploca japonica） was studied and applied in interspecific hybridization between tussah silkworm and camphor silkworm to improve economic traits of tussah silkworm through distant hybridization.Sperm cells of tussah silkworm were collected from copulatory pouches of female tussah silkworm moths after 40 to 60 min of intraspecific mating and were used to artificially inseminate the unmated tussah silkworm moths,which could lead to 61.5% of moths fertilized,70.5% of eggs fertilized,and 60.8% of eggs hatched.Sperm cells of camphor silkworm could be collected from copulatory pouches of female camphor silkworm moths after 40 to 75 min of intraspecific mating.By means of insemination with single moth＇s sperm and complementary insemination with single and multiple moths＇ sperm,hibernating eggs of camphor silkworm ♀×tussah silkworm ♂ and live embryos of tussah silkworm ♀×camphor silkworm ♂ were obtained respectively.This result indicated that a distant hybridization between Antheraea pernyi and Dictyoploca japonica can be conducted based on the newly established artificial insemination technology.
出处
《蚕业科学》
CAS
CSCD
北大核心
2011年第3期544-548,共5页
ACTA SERICOLOGICA SINICA
基金
辽宁省自然科学基金(No.20082130)
关键词
柞蚕
栗蚕
人工授精
远缘杂交
Antheraea pernyi
Dictyoploca japonica
Artificial insemination
Distant hybridization
