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GRP94表达在肺癌细胞对VP-16化疗耐药中的作用 被引量：2

Expression of GRP94 and its function in chemotherapy resistance to VP-16 in human lung cancer cell line SPCA1
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摘要 [目的]检测在Ca2＋拮抗剂A23187诱导下,人肺腺癌细胞SPCA1中GRP94的表达水平,并分析其表达在肺癌细胞对VP-16耐药中的作用。[方法]采用RT-PCR、免疫荧光细胞化学方法检测不同浓度A23187处理组细胞的GRP94核酸及蛋白表达,用MTT法测定细胞在VP-16作用下的生存率。[结果]A23187可明显诱导SPCA1细胞GRP94核酸和蛋白表达,且表达水平随A23187呈浓度依赖性增加。MTT法测定结果显示：诱导组细胞（A23187浓度为0.5~6μmol/L）对VP-16的IC50值明显高于对照组,而且IC50值的升高亦对A23187呈一定的浓度依赖性,当A23187浓度为6μmol/L时IC50值最大。[结论]GRP94的表达与肺腺癌细胞SPCA1对VP-16的耐药性有关,有针对性地抑制GRP94基因表达或功能可能成为肺癌治疗的新方法。 [Objective] To investigate the expression of GRP94 under the induction of A23187 and its function in drug resistance to VP-16 in human lung cancer cell line SPCA1.[Methods] RT-PCR and Immunofluorescence were employed in analyzing the expression of GRP94 at mRNA and protein in SPCA1 cells treated with A23187 at different concentration（0,0.5,1.0,1.5,2.0,4.0,6.0 μmol/L）.Cell survival rate following VP-16 treatment was determined with MTT assay.[Results] To a certain extent,the expression of GRP94 at both mRNA and protein level was significantly,exhibiting up-regulated by A23187 a dose-dependent manner.The mRNA expression of GPR94 was elevated up to 0.7~4.3 folds,compared to the control.The IC50s to VP-16 also showed a substantial elevation for the cells induced by A23187 at different concentrations.Survival curves analysis showed that the A23187 induction caused a significant prolonged survival for the cells subjected to VP-16（P0.05）.[Conclusions] Up-regulated GRP94 is associated with the chemoresistance of SPCA1 cells to VP-16.
作者 张黎川 王佳瑞 孟强 李恩成 王琪
机构地区 大连大学附属中山医院呼吸内科 大连医科大学研究生院 大连市第五人民医院肿瘤内科 大连医科大学附属第二医院呼吸内科
出处 《大连医科大学学报》 CAS 2011年第3期211-215,共5页 Journal of Dalian Medical University
基金 国家自然科学基金项目(30470464)
关键词 GRP94 人类肺腺癌系 VP-16耐药 GRP94 human lung adenocarcinoma VP-16 resistance
分类号 R734.2 [医药卫生—肿瘤]
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参考文献27

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二级参考文献25

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共引文献5

同被引文献19

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引证文献2

二级引证文献5

大连医科大学学报
2011年 第3期

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