摘要
目的了解北京市未经抗病毒治疗的HIV感染者耐药突变流行性情况。方法选取北京市2007年新确认HIV感染者抗凝全血标本150份，提取血浆中的病毒RNA，用反转录PCR和套式PCR扩增HIV的pol基因，并进行序列测定及耐药分析。结果成功扩增出111份标本的pol基因；未经抗病毒治疗的HIV感染者的耐药率为8．1％（9／111），蛋白酶抑制剂耐药率为3．6％（4／111），核苷类反转录酶抑制剂耐药率为1．8％（2／111），非核苷类反转录酶抑制剂耐药率为3．6％（4／111）。结论北京市未经抗病毒治疗的HIV感染者耐药性处于相对较高的水平，应当定期进行HIV耐药性监测。
Objective To examine the prevalence of drug resistance mutations among the treatment- naive HIV （human immunodeficiency virus） infectors living in Beijing so as to provide the basal information for clinical antiviral treatment. Methods HIV pol genes from plasma samples of 150 treatment-naive HIV- infected patients were amplified, sequenced and phylogenetically analyzed. And the drug-resistance associated mutations in protease and reverse transcriptase regions were analyzed with Stanford University HIV Drug Resistance Database. Results A total of 111 pol gene sequences were obtained. The overall prevalence of drug resistance was 8.1% （9/111 ）, corresponding to 3.6% （4/111 ） for protease inhibitors, 1.8% （2/111 ） for nucleoside reverse transcriptase inhibitors and 3.6% （4/111 ） for non-nucleoside reverse transcriptase inhibitors. No drug resistance mutation was identified in 17 intravenous drug users. Conclusion The prevalence of drug resistance is relatively high in the newly confirmed HIV infectors in Beijing. Regular surveillance and monitoring of drug-resistant HIV should be implemented.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2011年第21期1453-1456,共4页
National Medical Journal of China
