摘要
目的探讨喉癌组织中淋巴管生成与喉癌转移和预后之间的关系。方法采用免疫组化法对78例喉癌组织进行淋巴管内皮细胞透明质酸受体1（LYVE—1）染色，对LYVE-1标记的新生淋巴管进行计数，分析淋巴管密度（LVD）与喉癌患者临床病理特征以及预后的关系。结果LYVE-1标记的新生淋巴管主要位于肿瘤的边缘。在喉癌组织中，LYVE-1标记的LVD为13．24±5。09，明显高于成人喉乳头状瘤（5．54±3．15）和鳞状卜皮不典型增生（6．76±4．45，均P〈0．05）；而在转移的淋巴结中，LYVE-1标记的LVD为12．12±6．03，与喉癌组织中的差异无统计学意义（P〉0．05）。喉癌低分化组的LVD高于中分化组，中分化组的LVD高于高分化组；0～Ⅱ期组的IND显著低于Ⅲ～Ⅳ期；淋巴结转移组的LVD显著高于无淋巴结转移组（均P〈0．05）。喉癌组织的LVD与患者年龄、性别、临床分型和远处转移无关。LVD高于均值的喉癌患者的中位生存时间为33．5个月，低于均值者的中位生存时间为81．6个月，差异有统计学意义（P〈0．05）。Cox多因素生存分析结果显示，临床分期和LVD是影响喉癌患者预后的独立凶素（均P〈0．05）。结论喉癌组织中，LYVE-1标记的新生淋巴管主要位于肿瘤的边缘，淋巴管生成在喉癌发生、发展和淋岜结转移过程中起重要作用。IND是影响喉癌患者预后的负性因子。
Objective To detect lymphangiogenesis by labeling the lymphatic endothelial marker, lymphatic vessel endothelial hyaluronan receptor-1 （LYVE-1）, and study the prognostic relevance of lymphangiogenesis in laryngeal squamous carcinoma. Methods Clinical files and specimens of 78 patients with histologically diagnosed laryngeal carcinoma were stained with LYVE-1 as a specific lymphatic endothelial marker. The lymphatic vessel density （LVD） was measured, and the correlation between LVD and clinicopathologieal features of the tumor cases was analyzed. Results The mean LVD in laryngeal carcinoma （13.24 ±5.09） was significantly higher than that in adult laryngeal papilloma （5.54 ＋ 3.15 ） and squamous dysplasia （6.76 ±4.45, P 〈 0.05 ）. The LVD of poorly differentiated tumors （ 15.74 ±5.24） was significantly higher than that in the moderately differentiated tumors （13.84 ±6.20）, and the LVD in the moderately differentiated tumors was significantly higher than that in the well-differentiated tumors （ 11.68 ±6.34）. The LVD in stage 0 to stagc Ⅱ.group （ 10.66 ±5.70） was significantly lower than that in the stage Ⅲ to Ⅳ group （ 17.01 ±6.35 ）. The lymph node metastasis group （ 17.25 ±7.37 ） was significantly higher than non-lymph node metastasis group （ 8.60 ±5. 23, P 〈 0. 05 ）. There was no significant association between LVD and age, sex, primary site and distant metastasis. The overall survival in the patients with a LVD higher than the mean value was 33.5 month, and that of cases with a [ND lower than the mean value was 81. 6 month （P 〈 0.05）. The multivariate survival analysis showed that the clinical stage and LVD were independent prognostic factors of laryngeal cancer. Conclusions The LYVE-1 staining histochemistry demonstrates that the lymphangiogenesis occurrs mainly at the edge of the tumors, and lymphangiogenesis plays an important role in the carcinogenesis, cancer progression and lymph node metastasis in laryngeal cancer. LVD may be an independent indicator of poor prognosis of laryngeal cancer.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2011年第6期461-464,共4页
Chinese Journal of Oncology
基金
山东省优秀中青年科学家科研奖励基金（BS2010YY059）
关键词
喉肿瘤
鳞状细胞癌
淋巴管密度
淋巴管内皮细胞透明质酸受体1
预后
Laryngeal neoplasms
Squamous cell carcinoma
Lymphatic vessel density
Lymphatic vessel endothelial hyaluronan receptor-1
Prognosis
