摘要
目的观察上皮性卵巢癌细胞株及肿瘤组织microRNA-200c(miR-200c)的表达水平,探讨其可能的临床价值。方法采用Real-time RT-PCR技术比较人卵巢癌母细胞系(HO-8910)、卵巢癌高转移能力变异细胞株(HO-8910PM)及HO-8910细胞团簇中miR-141、miR-200c的表达差异。同法测定上皮性卵巢癌(n=83)、交界性卵巢肿瘤(n=13)及正常卵巢组织(n=8)中miR-141、miR-200c的表达,并分析卵巢癌不同临床病理特征下二者的表达差异。结果与HO-8910、HO-8910PM相比,HO-8910细胞团簇中miR-200c表达下调(P<0.05);正常卵巢组织、卵巢交界性肿瘤、卵巢癌中miR-141、miR-200c表达依次上调(P<0.05)。转移性卵巢癌、低分化卵巢癌及透明细胞卵巢癌中miR-200c表达下调(P<0.05)。miR-200c高表达患者预后优于miR-200c低表达者(P<0.05)。结论 miR-200c表达下调与卵巢癌进展相关,可能提示卵巢癌预后不良。
Objective To observe the expression of microRNA-200c （miR 200c） in ovarian cancer cell line and tissues, and to assess its possible clinical value. Methods Real-time PCR was used to analyze the expression of miR-200c and miR 141 in HO 8910 （human ovarian cancer cell line）, HO-8910PM （highly metastatic ovarian cancer cell line）, and HO-8910 cluster cells. We also determined the expression of miR-200c and miR-141 in 83 ovarian cancer tissues, 13 borderline ovarian tumors, and 8 normal ovary tissues; the expression was compared between tumors of different pathological characters. Results Expression of miR-200c was down-regulated in HO-8910 cluster cells compared with those in HO-8910 and HO-8910PM cells （P^0.05）. Expressions of miR-141 and miR-200c were gradually up-regulated from the normal ovary tissue to borderline ovarian tumors, then to ovarian cancer tissues （P%0. 05）. Expression of miR 200c was down regulated in the metastatic ovarian cancer and ovarian clear cell tumors and undifferentiated ovarian cancer cells（P%0.05）. Patients with high miR-200c expression had a better prognosis than those with a low expression of miR-200c （P% 0. 05）. Conclusion The decreased expression of miR-200c is correlated with the progression of ovarian cancer patients, and it is a risk factor of poor prognosis.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2011年第6期612-616,共5页
Academic Journal of Second Military Medical University
关键词
微RNAS
卵巢肿瘤
预后
microRNAs
ovarian neoplasms
prognosis
