摘要
目的观察川芎嗪对转化生长因子β1(TGF-β1)诱导的人肾小球系膜细胞增殖及Ⅳ型胶原分泌的影响。方法采用TGF-β1诱导肾小球系膜细胞增殖和Ⅳ型胶原表达以模拟肾脏疾病中的细胞纤维化,实验分为5组:空白组,对照组,川芎嗪高、中、低剂量(终浓度分别为100、30、10μg/ml)组。MTT法检测肾小球系膜细胞的增殖抑制率,ELISA法检测肾小球系膜细胞Ⅳ型胶原的表达。结果川芎嗪高剂量(100μg/ml)能够抑制TGF-β1诱导的肾小球系膜细胞增殖(P<0.01);川芎嗪可抑制TGF-β1诱导的肾小球系膜细胞中Ⅳ型胶原的表达,以高剂量组(100μg/ml)效果最为显著(P<0.01)。结论川芎嗪可抑制TGF-β1诱导的肾小球系膜细胞增殖及Ⅳ型胶原分泌。
Objective To observe the effect of ligustrazine hydrochloride（LHC） injection on TGF-β1-induced proliferation and type IV collagen secretion in the human mesangial cells（HMCs）. Methods The interstitial fibrosis in kidney disease was mimicked by inducing proliferation and type 1V collagen secretion in HMCs with TGF-β1. The experiment was divided into 5 groups： blank, control, low, medium, and high（10, 30, and 100 μg/ml）ligustrazine hydrochloride groups. MTT method was adopted to examine the proliferation and inhibition rate of HMCs. Enzyme-linked immunosorbent assay was used to determine the production of type IV collagen in cultured HMCs. ResuLts LHC at high concentration （100 μg/ml） significantly inhibited the proliferation of HMCs （P〈0. 01）. LHC also inhibited the production of type IV collagen, with the significant inhibition found when at the concentration of 100 μg/ml （P〈0.01）. Conclusion LHC can greatly inhibit TGF-β1-induced proliferation and expression of type IV collagen in human mesangial cells.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2011年第6期633-635,共3页
Academic Journal of Second Military Medical University
关键词
川芎嗪
肾小球系膜细胞
转化生长因子Β1
胶原Ⅳ型
ligustrazine hydrochloride
mesangial cells
transforming growth factorβ1
collagen type IV
