摘要
目的探讨白癜风维生素D(VDR)受体Apa I基因多态性及其与免疫异常的关系。方法采用聚合酶链反应和限制性片段长度多态性方法,对46例白癜风患者和50例健康人的VDR Apa I基因多态性进行分析,以及各基因型与外周血免疫球蛋白IgAI、gMI、gG,补体C3、C4以及T细胞亚群CD3+、CD4+、CD8+之间的关系。结果①VDR-Apa I位点的aa基因型在白癜风患者中出现的频率高于正常对照组(P<0.05)。②Aa、aa基因型组白癜风患者血清免疫球蛋白IgA水平显著低于正常对照组(P<0.05与P<0.01),aa基因型患者补体C3水平显著低于正常对照组(P<0.01)。③aa基因型组白癜风患者的CD3+T淋巴细胞百分比明显低于正常对照组(P<0.05)。结论携带aa基因型的白癜风患者存在体液与细胞免疫的异常。维生素D受体以及免疫异常与白癜风发病有一定的关系。
Objective To investigate the association between vitamin D receptor（VDR） Apa I gene polymorphism and immunological abnormality on vitiligo patients.Methods VDR genotypes of 46 vitiligo patients and 50 healthy controls were tested by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP）.We also measured the levels of immunoglobulin IgG,IgM,IgA and complements C3,C4 and T lymphocyte subpopulations CD3＋,CD4＋ and CD8＋ of peripheral blood.Results ①There was significant difference in genotype distribution of VDR-Apa I polymorphisms between vitiligo patients,whose frequency of aa is higher（P0.05）,and normal controls.② Vitiligo patients with Aa,aa had higher level of IgA than control cases,and the C3 measured in aa genotype showed the same trend（P0.01）.③ Compared with normal controls,the percentage of CD3＋ was significantly lower in vitiligo patients with aa genotype（P0.05）.Conclusion It suggests that VDR is a susceptible gene for involving in vitiligo,and aa is the susceptible genotype for vitiligo patients.Immunological abnormality may exist in vitiligo patients with aa genotype.Our study also indicates that the pathogenesis of vitiligo might have some connection with VDR gene and immunological abnormality.
出处
《中国中西医结合皮肤性病学杂志》
CAS
2011年第3期148-151,共4页
Chinese Journal of Dermatovenereology of Integrated Traditional and Western Medicine
关键词
白癜风
VDR基因
基因多态性
免疫异常
vitiligo
vitamin D receptor gene
gene polymorphism
immunological abnormality
