摘要
目的探讨海藻糖作为深低温冷冻保护剂对液氮保存的同种带瓣大动脉组织细胞caspase-3表达的影响。方法在液氮中冻存时间点分为12、15、18个月，依据冻存液不同分为5组，分别是：0．1mol／L DMSO（对照组），0．1mol／L海藻糖（实验组1），0．1mol／L海藻糖＋0．1mol／L DMSO（实验组2），0．2mol／L海藻糖＋0．1mol／L DMSO（实验组3），0．3mol／L海藻糖＋0．1mol／L DMSO（实验组4）。采用RT-PCR和Western Blot方法分别测定caspase-3的相对表达量。新鲜组作为阴性对照。结果在每个时间点下（P〈0．05），细胞凋亡最轻的是新鲜组，较好的是实验组2和实验组3，其次依次为实验组4、实验组1，凋亡最严重的是对照组。结论海藻糖和DMSO的联合运用能够很好的抑制caspase-3的表达。0．1mol／L海藻糖＋0．1mol／LDMSO和0．2mol／L海藻糖＋0．1moL／L DMSO能最大限度的抑制caspase-3的表达。
Objective To observe the expression of caspase-3 on the trehalose as cryoprotectant for preserving aortic valve homograft in liquid nitrogen. Methods The aortic valve homograft was divided into 5 groups, namely： 0. 1 mol/L DMSO （control group） , 0.1 mol/L trehalose （experimental group 1） , 0.1 mol/ L trehalose ＋0. 1 mol/L DMSO （experimental group 2） , 0.2 mol/L trehalose ＋0.1 mol/L DMSO （experimental group 3）, 0. 3 moL/L trehalose ＋0.1 mol/L DMSO （experimental group 4）. At the time of 12 months, 15 months and 18 months when preserved in liquid nitrogen, relative expression of caspase-3 of the aortic valve homograft was measured by RT-PCR and Western Blot. Fresh group was a negative control group. Results At the same time（ P 〈 0.05 ）, the expression of caspase-3 of fresh aortic tissue was slightest. The experimental group 2 was in accord with the experiment group 3, which was of a sort compare with the fresh group. The experimental group 4, which was worse than the experimental group 2 and 3, ranked above the experimental group 1. The worst was the control group. Conclusions The joint use of trehalose and DMSO could well in- hibit the expression of caspase-3. Moreover, 0.1mol/L trehalose ＋0.1 mol/L DMSO and 0.2 mol/L trehalose ＋0. 1 mol/L DMSO could maximize the inhibition of the expression of caspase-3.
出处
《国际外科学杂志》
2011年第6期386-389,共4页
International Journal of Surgery
基金
青岛市科技发展计划（No.06-2-2-6-nsh-1）
