摘要
为明确Harpins蛋白对大豆疫霉根腐病的抗病机制,对抗感不同大豆品种幼苗喷施Harpins蛋白,并测定喷施后120 h内大豆根系中苯丙氨酸解氨酶(PAL)的动态变化。结果表明:大豆喷施harpins蛋白后,根中PAL活性较对照增加,其中感病类型品种增加幅度较大;在喷施Harpins蛋白后120 h内,感病品种的PAL活性呈先增加后降低的趋势,并于喷施后72 h达到峰值,而抗病品种的PAL活性呈缓慢增加趋势。
The soybean resistance to Phytophthora megasperma could be significantly improved by spraying Harpins protein. In order to make clear the resistance mechanism of Harpins protein to Phytophthora megasperma, we sprayed Harpins protein on soybean seedling of different resistance soybean varieties, and measured the dynamic changes of phenylalnine ammonialyase （PAL） in soybean root after spraying within 120 hours later. The results showed that Harpins protein increased PAL activities of tested varieties at 72 h after spraying, with those in susceptible ones significantly higher than resistant ones. For susceptible varieties, PAL activity showed increasing-and-then-decreasing trend and peaked at 72 h after spraying Harpins protein; while the PAL activity showed slowly increasing trend in susceptible varieties.
出处
《大豆科学》
CAS
CSCD
北大核心
2011年第3期526-528,共3页
Soybean Science
基金
黑龙江省教育厅高职高专院校科研资助项目(11555059)
全国农业技术推广与服务中心品种安全性监测项目(2010203)
关键词
大豆
疫霉根腐病
Harpins蛋白
PAL酶
Soybean
Phytophthora megasperma
Harpins protein
pbenylalnine ammonialyase （PAL）
