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培养基组成对里氏木霉合成纤维素酶的影响 被引量:5

Effect of Medium Components on Cellulase Production by Trichoderma reesei
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摘要 研究了碳源种类及比例、氮源种类及比例、碳氮比和添加物等对里氏木霉合成纤维素酶的影响。里氏木霉以含纤维素质量比为4∶6的蒸汽爆破玉米秸秆和微晶纤维素为碳源,含氮素质量比为5∶2的硫酸铵-尿素为氮源,C/N为6,添加4 g/L黄豆粉和10 g/L麸皮为主要成分的培养基合成纤维素酶,纤维素酶活力在培养132 h达到最大,滤纸酶活力和β-葡萄糖苷酶活力分别为8.37 FPIU/mL和1.61 IU/mL。 Effects of different kinds and ratio of carbon sources,nitrogen sources and C/N ratio on biosynthesis of cellulase by Trichoderma reesei Rut C-30 were investigated.Production of cellulase was carried out using steam-exploded corn stover and Avicel as carbon sources(m(corn stover)∶m(Avice)4∶6),ammonium sulfate and urea as nitrogen sources(m(N of ammonium sulfate)∶m(N of urea)5∶2),soybean meal 4g/L,wheat bran 10g/L and the C/N 6.The results showed that the maximum filter paper activity and β-glucosidase activity were 8.37FPIU/mL and 1.61IU/mL,after cultivation for 132 h respectively.
出处 《林产化学与工业》 EI CAS CSCD 北大核心 2011年第3期55-59,共5页 Chemistry and Industry of Forest Products
基金 国家自然科学基金资助项目(30871992 31070523) 江苏省科技支撑计划项目(BE2010732) 林业公益性行业科研专项项目(201004001) 江苏高校科技创新团队项目资助(无编号)
关键词 里氏木霉 纤维素酶 培养基 Trichoderma reesei cellulase medium
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参考文献11

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