摘要
甘蓝型油菜不育系20118A为三隐性上位互作遗传模式,其不育性受两对重叠隐性不育基因(a和b)与一对隐性上位抑制基因(rf)互作控制。rf基因纯合时对a和b基因起抑制作用,使油菜育性恢复,可用于核不育三系法育种。为缩短临保系的育种周期,本文利用一个20118A与其临保系M-6029的BC1分离群体获得了7个与rf连锁的AFLP(扩增片段长度多态性)标记,构建了局部连锁图,图距在3cM～17cM。其中距rf最近(3cM)的标记为EA10MC03。用引物对F3和R3成功地将EA10MC03转化成SCAR(序列特异性扩增区)标记,能区分杂合型Rfrf单株和纯合型RfRf单株,为分子标记辅助选择隐性核不育临保系奠定了基础。
A recessive genic male sterility （RGMS） line 20118A was controlled by 2 recessive sterile genes （a and b） interacting with an inhibitor gene Uv. Gene Uc restored the fertility and was epistatic to a and b genes. The object of this study was to develop molecular markers linked to rf gene for marker - assisted selection of temporary maintainer line. By using a BC1 population from 20118A x M -6029, 7 AFLP （amplified fragment length polymorphisms） markers were obtained and made into a linkage map of if gene. The genetic distances ranged from 3cM to 17cM. Among them, EA10MC03 marker was 3cM to （gene. It was transferred into SCAR （sequence -character- ized amplified region） marker using primer pair F3 and R3, which could identify the heterozygous individuals （Rfrf genotype） from homozygous ones （ RfRf genotype）. The markers showed their ability in developing temporary maintainer lines in the future.
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2011年第3期216-220,共5页
Chinese Journal of Oil Crop Sciences
基金
国家863计划(2009AA101105)
农青年科技2008(01)
关键词
甘蓝型油菜
隐性核不育
隐性抑制基因
临保系
AFLP
SCAR
Brassica napus L
Recessive genic male sterility （RGMS）
Recessive inhibitor gene （rf）
Temporary maintainer
AFLP
SCAR
