摘要
目的建立高效的转基因兔和克隆兔的胚胎移植实验技术平台。方法通过外科手术移植的方法将受精胚胎、转基因胚胎和体细胞核移植胚胎分别移植到同步发情或异步发情的受体兔输卵管。结果移植受精胚胎、转基因胚胎和体细胞核移植胚胎的受体兔怀孕率分别为100％、66．67％、36.36％，产仔数分别为19、24及17只，仔兔的成活率分别为90.48％、92．31％、17．65％。出生仔兔与移植胚胎数的比率分别为43．75％、21．31％、8．54％。结论成功建立了高效的转基因兔和克隆兔的胚胎移植的实验技术平台。
Objective To establish an efficient platform of embryo transplantation for production of transgenic rabbits through virus transduction and somatic nuclear transfer approaches. Method Fertil- ized embryos, transgenic embryos, and nuclear transfer embryos were surgically transplanted into oviduct of synchronous or asynchronous estrus rabbits. Result After transplantation with fertilized embryos, transgenic embryos, and nuclear transfer embryos, the pregnant rates of surrogates were 100%, 66.67% and 36.36%, with the birth of 19, 24 and 17 pups. Survival rates of the pups delivered by recipients with fertilized embryos, transgenic embryos, and nuclear transfer embryos were 90.48%, 92.31% and 17.65%, meantime, the ratios of delivered pub to transferred embryos were 43.75%, 21.31% and 8.54%, respectively. Conclusion An efficient rabbit embryo transfer platform was successfully established, which would be the foundation of production of transgenic rabbits and cloned rabbits.
出处
《实验动物与比较医学》
CAS
2011年第3期172-177,共6页
Laboratory Animal and Comparative Medicine
基金
国家科技部“艾滋病和病毒性肝炎等重大传染病防治”科技重大专项课题（2009ZX10004-405）
关键词
兔
胚胎移植
超数排卵
受精卵胚胎
转基因胚胎
克隆胚胎
Rabbit
Embryo transplantation
Superovulation
Fertilized embryo
Transgenic embryo
Nuclear transfer embryo
