摘要
目的积累本中心F344大鼠自发肿瘤背景数据，为开展新药诱癌试验做准备。方法SPF级F344大鼠336只，雌雄各半，体重180-200g，本中心屏障环境下饲养，随机分为4个组，即分别于6、12、18和24个月4个时间点处死动物32、64、64，176只，称量动物心、肺、肝、脾、肾、肾上腺、胸腺、脑、睾丸（或卵巢）和附睾（或子宫）等器官并计算脏器系数，常规病理学取材制片后显微镜检查，观察统计4个时间点动物自发性肿瘤的类型和发病率。结果高发的肿瘤性病变主要包括睾丸间质细胞瘤、垂体腺瘤、单核细胞白血病、皮下纤维瘤、乳腺纤维腺瘤、嗜铬细胞瘤等。结论本实验结果丰富了现有F344大鼠自发性肿瘤的发病数据，为开展新药诱癌试验提供了背景资料。
Objective To establish the background database of spontaneous tumors in F344 rats bred at the National Beijing Center for Drug Safety Evaluation and Research. Methods Three-hundreds and thirty six F344 rats weighing 180-200 g, maintained in SPF condition were included in this study. They were routinely bred at the barrier facility of the center. After 6, 12, 18 or 24 months, the rats were euthanized at the number of 32, 64, 64 and 176, respectively. The rats were grossly examined and the weight of heart, lung, liver, spleen, kidney, adrenal gland, thymus, brain, testes （ovaries for the female） and epididymis （uterus for the female） were recorded and the coefficients of the organs were calculated. Tissues were routinely processed for paraffin sectioning and H.E. staining and histopathological examination were performed. The spontaneous tumors were classified and their incidence rates were calculated. Results The frequently found neoplasm in F344 rats include leydig cell tumor, adenoma in pituitary gland, large granular lymphoma （LGL）, subcutaneous fibroma, mammary adenoma and pheochromocytoma. Conclusion The present study provides the background database of the spontaneous tumors in the F344 rats, and may be laid a good foundation for the carcinogenesis studies for the new drugs.
出处
《实验动物与比较医学》
CAS
2011年第3期188-192,共5页
Laboratory Animal and Comparative Medicine
基金
国家科技部“重大新药创制”科技专项（2008ZX09305-003）资助.
关键词
大鼠
肿瘤
病理学
Rat
Carcinoma
Pathology
