摘要
目的通过动物模型的构建,为大肠癌研究提供可靠的实验依据。方法以二甲基肼诱发Wistar大鼠大肠癌,分别于给药后12、18、24周处死动物,观察各时期实验组及对照组大鼠大肠粘膜的病理变化。结果诱癌过程中大鼠大肠依次出现轻、中、重度不典型增生。诱癌12周时实验组未见肿瘤形成;18周肿瘤的发生率为60%,50%为大肠癌;24周肿瘤的发生率为100%,大肠癌的发生率为80%。结论二甲基肼诱导大鼠大肠癌成功率高,与人类大肠癌发生、发展相似,可用于模拟人类大肠癌的实验研究。
Objective To establish colorectal cancer animal model and provide experimental basis for human colorectal cancer research.Method Using dimethylhydrazine to induce Wistar rat colorectal cancer and killed them at 12,18,and 24 weeks after treated,the colorectal mucosa pathological changes of different experiment and control groups were observed.Results During the carcinogensis process,the light,medium and heavy atypical hyperplasia were observed.At 12 weeks,none cancer was observed in experimental groups.At 18 weeks,the incidence of tumor and colorectal cancer were 60% and 50% respectively.At 24 weeks,the incidence of tumor and colorectal cancer were 100% and 80% respectively.Conclusions The incidence of rat colon cancer model induced by dimethylhydrazine were high,the development of which was similar to the human colorectal cancer.The model can be used for the experimental study of human colorectal cancer.
出处
《医学动物防制》
2011年第6期519-520,F0003,共3页
Journal of Medical Pest Control
关键词
二甲基肼
大肠癌
大鼠
动物模型
Dimethylhydrazine
Colorectal cancer
Rat
Animal model
