摘要
目的研究白藜芦醇对人肺腺癌A549细胞周期的影响,并探讨其分子机制。方法 MTT法检测白藜芦醇对人肺腺癌A549细胞增殖的影响;PI单标流式细胞术检测白藜芦醇对A549细胞周期分布的影响;Western blotting法检测白藜芦醇对A549细胞cyclin D1和p21cip1蛋白表达的影响。结果 MTT法检测显示,白藜芦醇能明显抑制人肺腺癌A549细胞的增殖,其抑制作用呈时效和量效关系,100μmol/L白藜芦醇在作用48h时抑制率最高,为(76.54±1.33)%。流式细胞术检测提示不同浓度白藜芦醇作用24h细胞周期发生改变,G0/G1期细胞比例明显增加(P<0.01),且呈剂量依赖关系。Westernblotting法检测显示,白藜芦醇以时间依赖方式下调周期蛋白cyclin D1的表达,上调p21cip1蛋白表达。结论白藜芦醇能明显抑制人肺腺癌A549细胞的增殖。白藜芦醇可通过调节细胞周期蛋白cyclin D1、p21cip1的表达调控细胞周期进程,抑制细胞增殖。
Objective To investigate the anti-cancer activities of resveratrol on human lung adenocarcinoma A549 cells and its molecular mechanism involved.Methods The effects of resveratrol on the growth of human lung carcinoma A549 cell lines were studied by MTT assay.The effect of resveratrol on the cell cycle phase distribution of A549 cells was analyzed using flow cytometry by a propidium iodide method.The effect of resveratrol on the expression of cyclin D1 and p21cip1 protein was studied by Western blotting analysis.Results MTT assay showed that resveratrol could significantly inhibit the growth of human lung adenocarcinoma A549 cells.It inhibited the proliferation of A549 cells in a time and dose dependent manner,and the highest inhibitory rate was（76.54±1.33）% at the concentration of 100μmol/L when cells were cultured for 48h.Meanwhile,different concentrations of resveratrol treated A549 cells for 24h resulted in an increase of G0/G1 phase cells（P0.01）.The expression of cyclin D1 protein was decreased in a time dependent manner in A549 cells treated with resveratrol,while p21cip1 protein was increased.Conclusion Resveratrol could significantly inhibit the growth of A549 cells.Resveratrol is able to inhibit the proliferation of A549 cells by arresting the cell cycle at high concentrations.Regulating the levels of cell cycle regulatory proteins may suggest an involvement in the resveratrol-induced cell cycle arrest.
出处
《临床肿瘤学杂志》
CAS
2011年第6期506-509,共4页
Chinese Clinical Oncology
