期刊文献+

灯盏花素片在健康人体内的药代动力学研究 被引量:5

Pharmacokinetics of Breviscapine Tablets in Healthy Volunteers
原文传递
导出
摘要 目的:建立测定人血浆中灯盏花素浓度的高效液相色谱法。方法:色谱柱Kromasil C18(4.6 mm×250 mm,5μm);流动相甲醇-水-冰醋酸(30∶70∶1)检测波长:335 nm;灯盏乙素作为内标,用HPLC测定人血浆中灯盏花素浓度。结果:灯盏花素线性范围为0.30~18.0 mg·L-1,回归方程Y=0.008 7C-0.001 56(r=0.999 6);回收率>80%,日内和日间RSD均<10%。结论:方法简便、快速、准确,适用于灯盏花素药代动力学研究及血药浓度监测。 Objective:To establish a high performance liquid chromatographic method for determination the concentration of breviscapine in plasma of healthy volunteers.Method:The assay was conducted on the Kromasil C18(250 mm×4.6 mm,5 μm)columnn with methanol-water-glacial acetic acid(30∶70∶1)as the mobile phase.The detection wave length was set at 335 nm,and scutellarin was taken as the internal standard.Result:The linearity range was 0.30-18.0 mg·L-1 with a regressive equation of Y=0.008 7 C-0.001 56(r=0.999 6).The recovery was above 80%,with both intra-day RSD and inter-day RSD below 10%.Conclusion:The method was shown to be convenient,rapid,accurate,and suitable for the pharmacokinetic study of breviscapine and monitoring its concentration in clinical application.
出处 《中国实验方剂学杂志》 CAS 北大核心 2011年第13期110-112,共3页 Chinese Journal of Experimental Traditional Medical Formulae
关键词 灯盏花素 高效液相色谱法 药代动力学 breviscapine HPLC pharmacokinetics
  • 相关文献

参考文献6

二级参考文献34

共引文献26

同被引文献57

引证文献5

二级引证文献15

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部