摘要
为了解豫西南玉米穗腐病的病原菌,于2009年9月在河南省南阳市6个县区采集玉米穗腐病样品,进行病原菌的分离与单孢纯化。依据致病性、形态及分子检测结果进行病原菌鉴定。研究结果表明,分离得到的105株镰刀菌中,层出镰刀菌Fusarium proliferatum为70株,占分离镰刀菌的66.7%,属优势种群。在基于核糖体基因内转录间隔区（rDNA-ITS）与翻译延长因子1α基因（tef）序列构建的系统发育树中,3株代表菌株Fp1、Fp2和Fp3分别与GenBank登记的层出镰刀菌在自举值89%和91%水平相聚同一群。层出镰刀菌的生长温度为13~34℃,最适温度为28℃;在pH5.0和6.0各有1个生长高峰;碳源对该菌营养生长的影响较为稳定,而氮源对其营养生长影响的变幅较大。
To know the pathogens causing maize ear rot in southwestern Henan,the maize ear rot samples were collected from 6 counties in Nanyang City in September,2009.Pathogens were isolated from the samples and purified with single spore isolation.The pathogens were identified based on results of pathogenicity tests,morphological observations and molecular analyses.Seventy isolates were identified as members of Fusarium proliferatum,contributing about 66.7% of the Fusarium isolates obtained and indicating that F.proliferatum was a predominant Fusarium species causing maize ear rot in Nanyang.Two phylogenetic trees were established based on rDNA-ITS（internal transcribed spacer） and tef（translation elongation factor 1α gene） sequences,respectively.Three representative isolates Fp1,Fp2 and Fp3 clustered with GenBank-derived F.proliferatum isolates at 89% bootstrap level for rDNA-ITS sequences,and 91% for tef gene sequences.F.proliferatum could grow at temperatures of 13-34℃ with 28℃ as the optimum.Growth peaks occurred at pH 5.0 and pH 6.0,respectively.Vegetative growth of F.proliferatum was found to be significantly influenced by nitrogen sources compared to carbon sources.
出处
《植物保护学报》
CAS
CSCD
北大核心
2011年第3期233-239,共7页
Journal of Plant Protection
基金
河南省科技创新团队建设项目(2010JRTSTHNO12)
关键词
玉米
层出镰刀菌
致病性
鉴定
maize
Fusarium proliferatum
pathogenicity
identification
