摘要
目的明确黄芩的高效液相色谱(HPLC)图谱与解热药效的相关性,表征黄芩有效成分的药效物质基础。方法通过干酵母造大鼠发热模型,并给予黄芩提取物。用灰色关联统计方法将HPLC图谱的各组分峰的峰面积和解热药效数据相关联,研究谱效相关性,找出黄芩解热作用的物质基础。结果对于解热作用来讲,不可单一靠黄芩苷来表征,也应注意到黄芩素及其他黄酮类物质对解热作用有着不可忽视贡献。用灰色关联的分析方法,根据关联度大小来确定黄芩中以黄芩苷、黄芩素为代表的黄酮类物质对解热作用贡献程度。结论研究有效组分HPLC图谱特征及谱效关系,是表征中药—药效相关性的有效途径。
Objective To clear the correlation between HPLC spectrum and antipyretic effect of Huangqin （Radix Scutellariae ）, and to characterize the efficacy material basis of active principles of Huangqin. Methods The rat model of fever was established with dried yeast and then was given extractive of Huangqin. The peak areas of all component peaks in HPLC spectrum were connected to the data of antipyretic effect by using grey relation statistic method. The spectrum-effect correlation was studied and the material basis of Huangqin＇s antipyretic effect was found out. Results The antipyretic effect was not characterized only by baicalin, and the contribution of baicalein and other flavonoids should not be neglected. The contribution degrees of flavonoids in Huangqin like baicalin and baicalein were determined by using grey correlation analysis according to the correlation degrees. Conclusion It is an effective way for characterizing Chinese medicinal-efficacy correlation to study HPLC spectrum features and spectrum-effect relationship of effective components of Chinese medicinal.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2011年第6期379-383,共5页
Journal of Beijing University of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(No.30672642)
科技部基础工作专项资助项目(No.2001DEA2001O)
大连市科技局社会发展重点资助项目(No.2004B4SF121
No.2005E11SF215)
关键词
黄芩
高效液相色谱
解热
谱效关系
Huangqin （Radix Scutellariae ）
HPLC spectrum
antipyretic effect
spectrum-effectrelationship
