摘要
近十多年来,江苏沿海化工产业发展迅速,化工废水的长期排放对水生生态系统及人群健康构成潜在威胁。采用非洲猴肾细胞(CV-1)核受体介导的体外转录激活试验方法,对中国东部沿海A、B、C三市的6个水源地进行了拟雌激素活性调查研究。结果表明:C市2处水源水的有机提取物在枯水期、平水期和丰水期均无拟雌激素活性检出,水质较好;A市的水源水提取物在枯水期具有明显的拟雌激素活性;B市的3处水源水有机提取物在3个季节均表现出显著的拟雌激素活性;雌激素活性当量计算结果表明,点位B-3在枯水期的当量浓度高于毒性限值,对水生生物的内分泌系统易造成威胁,应引起有关部门关注。另外,研究结果显示,CV-1细胞受体转录激活试验是一种筛选和分析环境样品的具有拟雌激素活性的快速和有效的方法,值得进一步推广和应用。
Chemical industries have developed rapidly.Over the last decade,along the sea in Jiangsu Province,chemical wastewater discharged in long-term made potential threats to the ecosystem and human health.In this study,ER-mediated reporter gene assay was applied in evaluating the endocrine-disrupting activity of surface water from 6 water sources in 3 coastal cities of Jiangsu Province.The results indicated that source water in city C was the cleanest.Water extracts from city A in dry season could induce significant estrogenic activity.Extracts from water sources in city B could induce significant estrogenic activity in all three sampling seasons.Estrogenic activity equivalent at location B-3 in the dry season was higher than the indicated toxicity limit,which may pose a threat to the aquatic organism.More attentions should be paid to this part.This study also showed that reporter gene assay depending on CV-1 cell line was an efficient and rapid method for screening and analyzing of environmental samples,which worth further promotion and application.
出处
《生态毒理学报》
CAS
CSCD
2011年第3期241-246,共6页
Asian Journal of Ecotoxicology
基金
教育部高等学校博士学科点专项科研基金资助课题(No.200802841030)
国家重点基础研究发展计划(No.2008CB418102)
关键词
沿海化工园
饮用水源水
内分泌干扰
受体报告基因
coastal chemical industrial park
drinking water sources
endocrine disruptors
reporter gene assay
