摘要
目的探讨Ehrlich苏木精染液的pH值变化对组织染色的影响,寻求提高HE染色质量的方法。方法用2 mol/L NaOH和HC l分别处理Ehrlich苏木精染液,测定其pH值;观察组织切片在不同pH值苏木精染液中的染色效果。结果组织切片在低pH值染液中细胞核着色清楚,呈蓝色或深蓝色,核浆对比清晰,无苏木精沉渣附着;组织切片在高pH值染液细胞核着色欠清楚,呈灰蓝色或紫黑色,核浆对比不清晰,苏木精沉渣附着较多。结论 Ehrlich苏木精染液pH值变化对HE染色影响较大,稳定染液的pH值在较低水平可明显提高HE染色质量。
Objective To investigate the effects of pH value changes in Ehrlich haematoxylin staining solution on the tissue staining and to discuss techniques to improve the quality of HE staining.Methods Ehrlich haematoxylin staining solution was treated with 2 mol/L NaOH solution and 2 mol/L HCl solution respectively,then pH values were determined.The tissue staining effects at different pH values in Ehrlich haematoxylin staining solution were observed.Results The karyon of the tissue slices in low pH values staining solution was blue or navy blue,clearly against its background,and the staining of karyon and cytoplasm contrasted sharply without any haematoxylin residues clung to the tissue slices;on the other hand,the karyon of the tissue slices in high pH values staining solution was grey blue or purple black,not clearly agaisnt its background,and the staining of karyon and cytoplasm contrasted hazily,with many haematoxylin residues clung to the tissue slices.Conclusion The changes of pH value in Ehrlich haematoxylin staining solution have great effect on the HE staining of the tissue slices,and keeping the pH value of staining solution in a low level can improve the quality of HE staining.
出处
《解放军医药杂志》
CAS
2011年第3期24-26,F0003,共4页
Medical & Pharmaceutical Journal of Chinese People’s Liberation Army
