摘要
微生态制剂是发展绿色养猪业的关键,乳酸杆菌是目前应用的微生态制剂中最重要的有益菌组成成分之一。分离、鉴定并筛选出1株耐酸和耐胆盐能力较强的乳酸杆菌,命名为乳酸杆菌L5株。采用单因素法和正交试验设计法对其发酵培养条件进行了优化,发酵培养菌落总数可达8.25×108 cfu/mL。
Probiotics is the key to developing green pig industry.Lactobacillus is one of the most important beneficial bacterium components in the current application probiotics.One Lactobacillus strain which had strong acid and bile salts resistance was isolated,and it was identified and named Lactobacillus strain L5.The fermentation conditions in liquid culture were studied by single factor test and orthogonal test.Under optimal conditions,the living bacteria concentration reached 8.25×108 cfu/mL in the fermentation liquid.
出处
《现代农业科技》
2011年第11期332-333,335,共3页
Modern Agricultural Science and Technology
基金
2009年度安徽省高校省级教学质量与教学改革工程项目"<微生物学>精品课程"(教高〔2009〕9)
关键词
猪源乳酸杆菌
分离鉴定
发酵条件
优化
Lactobacillus from swine
isolation and identification
fermentation condition
optimization