摘要
目的了解武汉地区慢性丙型肝炎患者的HCV基因分型,为合理选择治疗方案及疗效预测提供理论依据。方法用PCR-反向点杂交技术对82份HCV-RNA阳性的血清进行基因分型,并对PCR产物测序验证,同时分析患者的性别、丙型肝炎临床类型与基因型分布相关性。结果 82份HCV感染样本共检出4种基因型,分别为lb、2a、3b和6a型,其中1b占73.1%,2a占14.6%,3b占7.3%,6a型占5.0%,各型测序结果与GenBank相应序列一致,1b型患者中重度型丙型肝炎发病率显著高于其他各型(P<0.05),基因型分布差异与性别无关。结论武汉地区HCV基因型主要为1b型,其次为2a型,与中国其他地区HCV基因型分布相一致,1b型患者临床症状较其他类型重,输血是丙型肝炎传播的主要途径,PCR-反向点杂交技术检测丙型肝炎病毒基因分型结果准确可靠、操作简便,具有临床推广和应用价值。
OBJECTIVE To investigate the distribution of hepatitis C virus （HCV） genotype in Wuhan, and to provide scientific basis for treatment program and prognosis. METHODS Totally 82 cases of HCV-RNA positive samples were typed by PCR-reverse dot blot （PCR-RDB）, and sequential analysis of the PCR products were done to identify the genetyping. Genotypes distribution was investigated in patients with different gender and clinical types of hepatitis C. RESULTS Four different HCV genotypes namely lb（73.1% ）, 2a（14.6 % ）, 3b（7.3 % ） and 6a （5.0%） were identified by PCR-RDB in 82 serum samples from HCV infected patients, The sequence of PCR products were identical to those reported in Genbank. Severe patients with 1b HCV had a higher prevalence than other genotypes. CONCLUSION HCV 1b is the major genotype distributed in Wuhan. The prevalent strains of HCV in Wuhan area are similar to those found in other regions in China. HCV genotype 1 b was associated with more serious clinical performance,the accurate genotyping results could be got by applying PCR-RDB.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2011年第13期2674-2676,共3页
Chinese Journal of Nosocomiology
