摘要
目的： 为探讨氨基胍（ＡＧ）对非酶糖化（ＮＧＥｓ）引起的人脐静脉内皮细胞信号（ＤＡＧ）转导的影响及其机制。方法：采用薄层层析、放射自显影及放射酶标法分离、检测细胞中ＤＡＧ含量，应用荧光法检测ＮＧＥｓ的含量。结果：氨基胍组荧光值从２７．８±５．９（ＡＦＵ）降为８．５±２．８（ＡＦＵ） ，ＤＡＧ含量从５４１．５±４６．２３ ｐｍ ｏｌ·Ｌ－ １ 降为２５３．５±１８．２０ ｐｍ ｏｌ·Ｌ－ １。结论：氨基胍明显阻断糖基化终末产物（ＮＧＥｓ）的形成，并且由ＮＧＥｓ刺激内皮细胞产生的ＤＡＧ含量显著降低。
Aim:To study the mechanism and effect of aminoguanidine(AG) on nonenzymatic glycosylation induced signal transduction in cultured human umbilical vein endothelial cells(HUVEC). Methods: Separation and quantitative measurement of diacylglycerol(DAG) were studied with thin layer chromatography,autoradiography and radioenzymatic assay. The formation of nonenzymatic glycosation end product(NGEs) was measured by fluorescence method. Results:In adding AG groups the AFU was decreased from 27.8±5.9 to 8.5±2.8. The levels of DAG was also decreased from 541.5±46.23 pmol·L -1 to 253.5±18.20 in HUVEC. Conclusion: The results suggest that AG significantly inhibit the formation of NGEs. AG also markedly attenuated the accumulation of DAG .It is an important theoretical basis to prevent chronic complications in diabetic mellitus.
出处
《高血压杂志》
CSCD
1999年第4期377-379,共3页
Chinese Journal of Hypertension
关键词
氨基胍
非酶糖化
信号转导
内皮细胞
二酰基甘油
aminoguanidine
\ nonenzymatic glycosylation
\ signal transduction
\ endothelial cells
\ diacylglycerol
