摘要
利用正相液相色谱-飞行时间质谱建立了大鼠血浆磷脂的轮廓谱.通过对磷脂轮廓谱的偏最小二乘判别分析(PLS-DA)及14种化合物的定量测定,考察了中药糖肾方对自发性Ⅱ型糖尿病鼠磷脂代谢的影响,并对比了糖肾方与西药蒙诺在糖尿病发展过程中的不同作用.结果表明,与正常对照组相比,糖尿病鼠的血浆磷脂代谢发生异常.糖肾方能够调节糖尿病鼠的磷脂代谢紊乱,蒙诺则加剧了病鼠的磷脂代谢异常.此外,8种磷脂化合物可能成为糖尿病发展过程中的潜在生物标志物.
As one of the most common endocrine diseases,diabetic mellitus（DM） has a very close relationship with phospholipids metabolism.Tangshen Formula（TSF） is an often-used prescription with good efficacy for treating DM and diabetic nephropathy（DN）.The aim of this study is to understand the effect of TSF on phospholipids metabolism of DM.In this paper,Otsuka Long-Evans Tokushima Fatty（OLETF） rats,a model of slowly progressive obese type Ⅱ diabetes,were employed.Phospholipids profiling of rat plasma was established using normal phase liquid chromatography-time of flight mass spectrometry（NPLC-TOF/MS）.Partial least-squares discriminant analysis（PLS-DA） and quantification of 14 compounds were applied to evaluate the effect of TSF on phospholipids metabolism of OLETF rats and to discover potential biomarkers.The results showed that plasma phospholipids metabolic disorders occurred in OLETF rats.The good effect of TSF on phospholipids metabolism was compared with the adverse impact of Monopril.TSF could significantly improve phospholipids metabolism of OLETF rats,whereas Monopril exacerbated disturbance of phospholipids metabolism.According to the quantitative results,a total of 8 phospholipids compounds which have significant concentration variation between control group and model group were considered as potential biomarkers of type Ⅱ diabetes.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2011年第7期1512-1518,共7页
Chemical Journal of Chinese Universities
基金
国家自然科学基金(批准号:90707045,20805026,90917003)
国家“九七三”计划项目(批准号:2005CB523503,2007CB714505)资助
关键词
糖肾方
OLETF大鼠
磷脂代谢
NPLC-TOF/MS
偏最小二乘判别分析(PLS-DA)
Tangshen Formula
OLETF Rat
Phospholipids metabolism
Normal phase liquid chromatography-time of flight mass spectrometry（NPLC-TOF/MS）
Partial least-squares discriminant analysis（PLS-DA）
