摘要
目的:探讨玉米水溶性多糖(WSPC)对糖尿病大鼠胰岛免疫损伤修复的影响。方法:用链脲佐菌素(STZ)腹腔注射复制糖尿病大鼠模型,将造模成功的大鼠随机分成为低、中、高剂量组和模型对照组,连续灌胃28d后,观察WSPC对糖尿病大鼠的血糖、胰岛素(Ins)及β细胞功能指数(HOMA-IS)、谷氨酸脱羧酶抗体(GAD-Ab)、胰岛细胞抗体(ICA)、人胰岛素自身抗体(IAA)的含量变化。结果:与模型对照组相比,低、中、高剂量组血糖均显著性降低(P<0.05),存在剂效关系;Ins各组之间均有显著差异(P<0.05),并随着剂量的增加胰岛素水平逐渐增加,并接近正常对照组;HOMA-IS各剂量组与正常对照组均有显著差异,各剂量组之间并无显著差异;相对于模型对照组,随着剂量的增加,3个抗体指标都有所下降,其中GAD-Ab和IAA的高剂量组与模型对照组存在显著差异,ICA的中、高剂量组与模型对照组之间有显著差异。结论:WSPC能促进胰岛β细胞分泌胰岛素,显著提高血清胰岛素水平。WSPC通过修复自身免疫损伤,促进胰岛素分泌,有显著的降低血糖作用。
Objective：To explore the repairing effect of water-soluble polysaccharide from corn（WSPC） on islet immune damage in diabetic rats.Methods：Streptozotocin（STZ） was used to establish diabetic rat models via intraperitoneal injection.The successfully established models were randomly divided into three groups administered with WSPC at low,middle and high doses,as well as the model control group.WSPC administration was carried out by gavage for 28 consecutive days.The changes in blood sugar index,INS,HOMA-IS,and the contents of GAD-Ab,ICA and IAA in the diabetic rats were determined.Results：Compared with the model control group,blood glucose levels in treatment groups administered with WSPC at the low,middle and high doses were significantly decreased in a dose-dependent manner（P0.05）.Meanwhile,INS was increased with increasing WSPC dose and revealed a significant difference between the three treatment groups and the model control group（P0.05）.Compared with the normal control group,HOMA-IS in each WSPC group had a significant difference;however,no obvious difference in each WSPC was observed.In addition,compared with the model control group,GAD-Ab and IAA in the high-dose group did not exhibit an obvious difference;in contrast,ICA revealed a significant difference betweeb the middle-dose and high-dose groups.Conclusion：WSPC can promote insulin secretion in pancreatic islet β cells through rehabilitation of autoimmune injury and significantly increase serum insulin level（P 0.05）,thereby playing an important role in reducing blood glucose level.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第13期302-305,共4页
Food Science
基金
广东省引进人才基金项目(201192)
广东石油化工学院人才引进基金项目(2010r2)
关键词
多糖
糖尿病
键脲佐菌素
抗体
胰岛细胞
polysaccharides
diabetes
streptozotocin
antibody
islet cell
