摘要
目的 观察EB病毒 (Epstin Barrvirus ,EBV)、抑癌基因P5 3、P16在鼻、鼻咽和扁桃体非霍奇金淋巴瘤 (non Hodgkin’slymphoma ,NHL)中的表达 ,并分析其相互间关系。方法 用原位分子杂交检测 3 6例病变组织的EBV DNA、P16mRNA ,用免疫组化技术检测LMP1、P5 3及P16蛋白 ,5例原位杂交检测为EBV阴性的病例用原位PCR检测EBV DNA。结果 ① 2 3例 (63 9% )检出EBV DNA ,11例(3 0 6% )检出P16mRNA ;② 10例 (2 7 8% )LMP1阳性 ,7例 (19 4 % )P5 3蛋白阳性 ,8例 (2 2 2 % )P16蛋白阳性 ;③T NHL(16/19例 )明显高于B NHL(6/17例 )的EBV DNA阳性表达。结论 鼻、鼻咽和扁桃体NHL尤其是T NHL有较高比例的EBV感染 ,但未发现EBV感染与P5 3及P16表达之间有明显相关性。
Objective To observe the presence of Epstin Barr virus(EBV) and expression of P53 and P16 in the non Hodgkin's lymphoma(NHL) occurring in the nasal cavity and pharyngeal Waldeyer's ring Methods In situ hybridization was used to detect EBV DNA and P16 mRNA and immunohistochemical staining was used to examine the expression of LMP1,P53 and P16 in the 36 cases Five cases whose EBV DNA were negative at first assay by hybridization was re detected for EBV DNA by PCR Results Positive expression rates of EBV DNA, LMP1, P16mRNA, P53 and P16 were 63 9%, 27 8%, 30 6%, 19 4% and 22 2% P53 and P16 expressions were not significantly different between T cell NHL and B cell NHL, nor were them significantly different between EBV positive and negative cases Conclusion A higher percentage of EBV infection was detected in the nasal cavity and pharyngeal Waldeyer's ring in NHL EBV infection was not significantly associated with P53 and P16 expresions in NHL
出处
《中华耳鼻咽喉科杂志》
CSCD
1999年第6期359-361,共3页
Chinese Journal of Otorhinolaryngology
关键词
非霍奇金淋巴瘤
鼻咽肿瘤
EB病毒感染
P53
P16
Lymphoma
non Hodgkin's
Genes
suppressor
tumor
In situ hybridization
Herpesvirus 4
human
