摘要
目的：观察偏头痛模型大鼠硬脑膜、三叉神经节及脑干区丝裂原活化蛋白激酶家族c-Jun氨基末端激酶-1（JNK-1）和G-蛋白耦联受体家族蛋白酶激活受体-2（PAR-2）的表达以及中药头痛宁对其影响。方法：成年SD大鼠30只随机均分为正常对照组、模型组和头痛宁治疗组3组,每组10只。模型组、治疗组大鼠皮下注射硝酸甘油注射剂10mg/kg建立实验性偏头痛模型,模型组造模30min后蒸馏水1.04mL/kg灌胃,治疗组造模15~20min后头痛宁1.2g/kg灌胃。1周后用免疫组化法检测各组大鼠硬脑膜、三叉神经节及脑干区JNK-1和PAR-2的表达。结果：模型组硬脑膜、三叉神经节及脑干区JNK-1和PAR-2的表达均高于其他2组（JNK-1：F硬脑膜=288.665,F三叉神经节=20016.775,F脑干=333774.771,P均〈0.001;PAR2：F硬脑膜=25790.690,F三叉神经节=820.497,F脑干=198.165,P均〈0.001）,治疗组和对照组上述指标差异无统计学意义（P〉0.05）。结论：头痛宁可通过下调偏头痛模型大鼠硬脑膜、三叉神经节及脑干区JNK-1和PAR-2的表达,防治偏头痛。
Aim： To observe the effect of on Chinese medicine Tou Tong Ning expressions of mitogen-activated protein kinase family,c-Jun N-terminal kinase-1 （JNK-1） and G-protein-coupled receptor family of protease-activated receptor-2 （PAR-2） in the dura mater,trigeminal ganglia and brainstem areas of migraine model rats induced by nitroglycerin.Methods：30 adult SD rats were randomly divided into 3 groups （n=10）：the normal control group,model group and Tou Tong Ning treatment group.Model group and treatment group were injected subcutaneouly with nitroglycerin 10 mg/kg to establish migraine model.Gastric perfusion of distilled water （1.04 mL/kg） was given in the model group after model establishment 30 min,and Tou Tong Ning（1.2 g/kg） was given in the treatment group after model establishment 15～20 min.JNK-1,PAR-2 expression were observed in the dura,the trigeminal nerve Festival and brainstem areas by immunohistochemical staining of each group.Results： In the model group,JNK-1,PAR-2 expression in more than the other three groups were significantly increased （JNK-1：Fdura mater=288.665,F trigeminal ganglion=20016.775,Fbrain stem=333774.771,P0.001;PAR2：F dura mater=25790.690,F trigeminal ganglion=820.497,Fbrain stem=198.165,P0.001）.Treatment group and the normal control group had no statistical significance （P0.05）.Conclusion：The Tou Tong Ning can prevent migraine headache through decrease the expression of JNK-1,PAR-2 in dura mater,trigeminal ganglia and brain stem areas of migraine model rats.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2011年第3期409-412,共4页
Journal of Zhengzhou University(Medical Sciences)
