摘要
目的探讨抗凝剂乙二胺四乙酸二钾（EDTA-K2）所致血小板聚集时血小板及白细胞计数结果的影响及纠正。方法对17例EDTA—K2依赖性血小板饭性减少症（PTCP）患者的EDTA—K2抗凝血分别于0，5，15，30min测定血小板（PLT）和白细胞（憾），另外对同时抽取的新鲜血（不加抗凝剂）即时上机测定和手工法潮定PLT和WBC；均取上述标本进行涂片染色显微镜镜检。结果EDTA—K2抗凝血PLT计数检测结果随待测时间延长越来越低，5，15min和30min时测定的PLT数量与0min时测定的血小板数量比较差异有统计学意义；5min和15min时测定的WBC数与0min时测定的W13（2数量差异无统计学意义（P〉0．05），30min时测定的W13（2数少于0min时测定的W1312数量（P〈0．01）；EDTA—K2抗凝血混匀后0min时测定的WBC和PLT结果与新鲜血（不加抗凝刺）仪器法、手工法测定的结果差异无统计学意义（P〉0．05）。结论EDTA—K2引起PTCP患者的血小板聚集导致血小板假性减低和白细胞假性增高；在临床检验工作中应加强镜检复核，对于首次检测血小板数值减低的标本均应涂片镜检，以确认是否存在抗凝剂所致血小板聚集的现象。对PTCP患者应采用新鲜血（不加抗凝剂）仪器即测法、手工法或EDTA—K2抗凝血混匀后即测等方法检测。
Objectives To discuss the effect platelet aggregation induced by anticoagulant EDTA-K2 disturb platelet counts and WBC counts,then correct it. Methods The level of platelet counts and WBC counts in 17 patients with PTCP was measured to anti-clotting blood at 0,5,15 and 30 min,besides the level of platelet counts and WBC counts of fresh whole blood （no anticoagulant） was measured by automatic blood cell analysing instrument and manual counting. The smears made by the above sample were viewed under a Inicroscope also. Results The level of platelet counts measured were lower by time longer to anticoagulants EDTA-K2 ,Results measured at 5,15 min and 30 min were lower than results measured at 0 min and the differences had statistical significance; The level of WBC counts measured at 30 min were lower than results measured at 0 min（P〈0.01）. The level of platelet counts and WBC counts was measured to anti-clotting blood at 0 min hadn＇t difference than it measured to fresh whole blood （no anticoagulant） by automatic blood cell analysing instrument and manual counting（P〈0. 05）. Conclusion Platelet aggregation in patients with PTCP caused false platelet counts false reduce and WBC counts false increase. Review under a microscope should strengthen in clinical laboratory work. Manual platelet counts or smear microscopy examination should be done on blood samples which platelet reduction should be discovered for the first time to confirm the phenomenon of platelet aggregation induced false platelet reduce. The patients with PTCP was determined by measuring the fresh whole blood（no anticoagulant） by automatic blood cell analysing instrument immediately,manual counting or measuring anti-clotting blood immediately.
出处
《现代检验医学杂志》
CAS
2011年第3期136-138,共3页
Journal of Modern Laboratory Medicine
