摘要
从河北某兔场单卵囊分离肠艾美耳球虫并接种无球虫兔进行增殖,CTAB法提取肠艾美耳球虫卵囊基因组DNA。利用艾美耳属球虫18SrDNA保守引物,PCR扩增肠艾美耳球虫18SrDNA片段,产物纯化后测序。测得的序列用DNAstar软件分析并与GenBank公布的11种兔球虫(EF694007-EF694017)的相应序列进行同源性分析,并绘制系统进化树。结果表明,扩增出的18SrDNA片段大小为1 521bp。序列分析显示,肠艾美耳球虫河北株18SrDNA与GenBank公布的11种兔球虫相应序列同源性为92.6%～99.9%,肠艾美耳球虫河北株与国外肠艾美耳球虫(EF694012)18SrDNA相似性达99.9%。系统发育进化树显示,肠艾美耳球虫河北株与肠艾美耳球虫(EF694012)亲缘关系最近。
Using single-oocyst seperation technology,E.intestinalis was isolated from rabbit in Hebei,then inoculated coccidia-free rabbits to propagate.Its genomic DNA was extracted by the method of CATB.Using conservative primer of 18S rDNA of Eimeria,18S rDNA gene fragment of E.intestinalis HB was amplified and sequenced.Among E.intestinalis HB and 11 species of rabbit-infecting Eimeria in the GenBank,the phylogenetic tree was constructed based on their 18S rDNA sequences by DNAstar software.The amplification results indicated that the gene fragment was amplified with 1 521 bp.The analysis of the percent identity showed that E.intestinalis HB shared the homology of 92.6%-99.9% with 18S rDNA sequence of 11 species of rabbit infecting Eimeria.The homology between E.intestinalis HB and E.intestinalis（EF694012） is 99.9%.The tree of phylogenetic analysis show that E.intestinalis HB and E.intestinalis（EF694012） are the most close.
出处
《西北农业学报》
CAS
CSCD
北大核心
2011年第5期31-34,共4页
Acta Agriculturae Boreali-occidentalis Sinica
基金
国家兔产业技术体系项目(nycytx-44)
