摘要
目的检测晚期肿瘤患者外周血树突状细胞数量和T细胞亚群的表达,评价肿瘤患者的免疫功能。方法应用流式细胞术检测49例晚期肿瘤患者外周血树突状细胞亚群的数量,免疫表型分别为:髓样树突状细胞,CD85K+CD33+CD(16+14)-;浆细胞树突状细胞,CD85K+CD123+CD(16+14)-;以及T淋巴细胞亚群的表达;以11例同年龄段健康人群作为对照组。结果肿瘤患者外周血中的髓样树突状细胞和浆细胞样树突状细胞的含量显著低于对照组(P<0.05,P<0.01);CD3+的表达低于对照组,但差异无统计学意义;CD3+/CD4+的表达高于对照组(P<0.05),CD3+/CD8+的表达低于对照组(P<0.05)。结论中、晚期肿瘤患者外周血中树突状细胞数量的减少和T淋巴细胞亚群的变化,可能是中、晚期肿瘤患者机体免疫功能低下的原因之一,本实验研究为临床肿瘤生物治疗提供了一定的实验依据。
Objective To determine the amount of dentritic cells（DC） and the subtypes of T lymphocytes in the peripheral blood to evaluate the immune function in patients with tumors at advanced stage. Methods Peripheral blood samples were collected （49 patients and 11 healthy controls） and examined for the subtypes of DC,including PDC,with immunophenotype of CD85K＋ CD123＋ CD（16 ＋ 14）- and MDC, with CD85K＋ CD123＋ （16 ＋ 14） , and of T lymphocytes. Results The levels of MDC, PDC and CD3＋/CD8＋ of patients group were significantly lower than those of control group（P〈0.05 ,P〈0.01） ,but level of CD3＋/CD4＋ of patients group was significantly higher than control group（P〈0.05）. There was no statistical difference of the level of CD3＋ cells between the two groups（P〈0.05）. Conclusion The decrease of the amount of DC and the change of the subtypes of T lymphocytes in the peripheral blood may be correlated with the hypofunction of the immune system of patients with tumor at medium stage or with advanced tumor.
出处
《国际检验医学杂志》
CAS
2011年第10期1053-1054,共2页
International Journal of Laboratory Medicine
关键词
肿瘤
树突细胞
细胞免疫
neplasms
dendritic cells
cell immunity
