摘要
[目的]研究具有好氧反硝化特性的异养硝化细菌NYMO和NYTE同时进行硝化和反硝化作用的能力及好氧反硝化菌HWTT的产物N2O逸出量情况。[方法]通过NH4+-N和NO3--N的质量浓度的变化情况来分析细菌硝化作用和反硝化作用的能力,采用空气密封摇瓶培养和额外补充O2保证好氧条件的2种方式培养菌株HWTT。[结果]菌株NYMO和NYTE 2 d内NH4+-N去除率为34.60%和35.08%,NO3--N去除率为33.40%和99.92%[结论]NYMO和NYTE具有异养硝化好氧反硝化能力。缺氧条件下,N2O逸出量最高值为0.15μmol,N2O/(N2O+N2)低于0.15%;好氧条件下,N2O逸出量为35.71μmol,N2O/(N2O+N2)为70%以上。
[Objective] The ability in the simultaneous nitrification and denitrification of the heterotrophic nitrifiers bacteria-NYMO and NYTE with the characteristics of aerobic denitrification and the amount of the product-N2O of the aerobic denitrifion-HWTT was researched.[Method] The capability in the nitrification and denitrification of the bacteria was tested through the analysis of the change of the concentration of NH4＋-N and NO3——N.The bacteria strain—HWTT was cultured with 2 culturing ways： air-sealed flask culture and aerobic culture based on the additional supplementary of O2.[Results] The removal rate of the NH4＋-N of the strain-NYMO and NYTE within 2 days was 34.60% and 35.08%,and the removal rate of NO3——N was 33.40% and 99.92%,respectively.[Conclusion] The bacteria-NYMO and NYTE was with the ability in both heterotrophic nitrification and aerobic denitrification.Under hypoxic condition,escape the highest value of N2O producing was 0.15 μmol and the ratio of N2O/（N2O＋N2） was less than 0.15%;and under the aerobic condition,the amount of N2O producing was 35.71μmol and the ratio of N2O/（N2O＋N2） was above 70%.
出处
《安徽农业科学》
CAS
北大核心
2011年第16期9765-9767,9796,共4页
Journal of Anhui Agricultural Sciences
基金
中央高校基本科研业务费专项资金(No.2009ZM0034)
广东省团队联合基金(No.9351064101000001)
关键词
脱氮微生物
异养硝化-好氧反硝化
N2O
C2H2
Denitrification microbes
Heterotropnic nitrification-aerobic denitrification
N2O
C2H2
