摘要
以变叶金银木为外植体,探讨了不同生长调节物质对外植体分化、增殖和生根的影响。结论如下：（1）变叶金银木组织培养最适宜的外植体是半木质化带芽枝段,以70%CH2（OH）560 s＋0.1%HgCl2消毒4~5 min为最好;（2）在不添加任何激素的MS培养基中20 d后能诱导出幼芽,适宜的增殖培养基为MS＋6-BA 0.3 mg.L-1＋NAA 0.1 mg.L-1,生根培养基为1/2 MS＋IBA 0.2 mg.L-1;（3）生根试管苗适宜的开瓶锻炼时间为3 d,选择直径为0.5~1 mm的试管苗移栽到蛭石培养基上能够获得76.7%~86.6%的成活率。
Using Lonicera maackii＇Bian ye ＇as explants,influences of different plant growth hormones on differentiation,multiplication and rooting were examined.The results show that（1）the optimal explant material was tender stem with single bud,sterilized 1 minute in 70% CH2（OH）5 and 4～5 minutes in 0.1% HgCl2;（2）the young buds cound be induced after 20 days cultured on the medium MS,the optimal subculture and rooting medium were MS＋6-BA 0.3 mg·L-1＋NAA 0.1 mg·L-1and 1/2 MS＋IBA 0.2 mg·L-1 respectively;（3）the glass seedling rooting condign open the glass bottle hardened times was 3 days,than the diameter 0.5~1 mm glass seedling was transplanted on vermiculite,the survive rates of glass seedling were 76.7%～86.6%.
出处
《中南林业科技大学学报》
CAS
CSCD
北大核心
2011年第6期116-121,共6页
Journal of Central South University of Forestry & Technology
基金
2009~2010北京农业职业学院国家级示范校建设科技研发项目(技术攻关与重点支持基金项目)"变叶金银木的发现与快繁技术研究"(No:SF-YF-09-08)
关键词
组织培养
外植体
增殖
变叶金银木
tissue culture
explant
proliferation
Lonicera maackii ＇Bian ye＇
