摘要
当年生栀子枝条插入水中诱导出不定根,分别进行溶液静止培养和土壤培养。60 d后测定生物量和相关生理指标,并对根、茎、叶横切面进行显微观察。结果表明,水培栀子生物量低于土培栀子的,含水量增加。与土培叶片较厚、较长相比,水培叶片变薄、变短、变宽;水培叶片表皮细胞大于土培叶片;与土培叶片柱状细胞整齐排列的栅栏组织相比,水培叶片缺乏柱状细胞,代之以球状小细胞无规则排列,且有较大的细胞间隙;水培叶片叶绿素b质量分数显著低于土培叶片的,表明水环境下植物对光能吸收有所下降。叶绿素a质量分数、过氧化物酶活性以及硝酸还原酶活性在2种条件培养叶片中差异不显著。水培栀子茎维管较土培栀子稍粗。2种培养条件下植物根系形态结构和生理指标差异显著:水培栀子白色或淡绿色根系比土培栀子褐色多毛的根系长1/3以上,有二级根和少量三级根,缺乏根毛;水培栀子根皮层细胞排列较疏松,部分细胞解体形成通气组织,而土培栀子根皮层细胞排列较致密,没有通气组织;水培栀子根系硝酸还原酶活性高于土培,呼吸强度则低于土培,表明栀子适应水生低氧环境所表现的分解代谢下降和氮代谢增强。
Newborn branches of Gardeniajasminoides Ellis were cut and put into water to induce adventitious roots. The branches were then divided into two groups to be cultured in static nutrient solution and soil respectively. The biomass and physiological indexes of G. jaminoides were determined after 60d of culture. The cross sections of root, stem and leaf were observed by microscope. Results show that, compared with the soil culture, the biomass of the hydroponic plants decreases and the water content increases. The leaf blade of the soil cultivated plants is thick and long, while that of the hydroponic plants becomes thinner, shorter and wider with larger epidermal cells. The columnar mesophyll cells of the soil cultivated plants arrange in palisade tissue, while the mesophyll cells of the hydroponic plants are spherical and smaller without palisade tissue but with larger intercellular space. Leaves from the hydroponic cuhnre have less chlorophyll b content than those from the soil culture, indicating the decrease in light absorption of the hydroponic plants, ttowever, the differences in chlorophyll a content, activities of Peroxidase （POD） and Nitrate Reductase （NR） are unobvjous between the two cultivation methods. Vascular of the stem of the water cultivated plants is thicker. Significant differences in morphological structure and physiological indexes of the root system were observed between the hydroponic and soil cultivated plants. Compared with the short brown hairy roots of the soil cultivated plants, the roots of the hydroponic plants, with secondary roots and fewer tertiary roots but without root hair, are white or light green and about one-third longer. Microscopic＇ally, the cortical cells in roots of the hydroponic plants loosely arrange with the lysis of some cortical cells resulting in the aerenehyma formation, but those cells in roots of the soil cultivated arrange tightly without aerenchyma. The more active nitrate reduetase and the decreased respiratory rate of the hydroponic plants show that G.jasminoides could adapt to low oxygen levels in water by inhibiting catabolism and enhancing nitrogen metabolism.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2011年第7期19-21,共3页
Journal of Northeast Forestry University
基金
国家自然科学基金项目(30771755)
黄山学院引进博士启动项目(2007xkjq028)
关键词
栀子
水培
显微结构
生理指标
Gardenia jasminoides
Hydroponic culture
Microscopic structure
Physiological indexes
